APG8 Control Lysate | Rockland Immunochemicals W00-001-P09 product information

product summary

company name :

Rockland Immunochemicals

product type :

other

product name :

APG8 Control Lysate

catalog :

W00-001-P09

quantity :

200 µg

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

W00-001-P09

Name :

APG8 Control Lysate - W00-001-P09

Display Name :

APG8 Control Lysate

Application Note :

Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). If dissociating conditions are desired, add reducing agent prior to heating. Gently invert vial several times to ensure that the solution is homogeneous and that any precipitated material is re-dissolved. A loading volume of 0.25 to 2.0 µL is recommended for SDS-PAGE. The product is supplied in 1X loading buffer and is ready-to-use.

Buffer :

1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)

Concentration Value :

1.0 mg/mL

Concentration Definition :

by modified Lowry assay

Conjugation :

(None)

Size :

200 µg

Default Unit :

µg

Western Blot Dilution :

User Optimized

Expiration :

Expiration date is three (3) months from date of opening.

Gene Name :

ATG8

Host Animal :

Other - E.coli

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Induction :

None (Control)

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Purity and Specificity :

Following induction for expression of the recombinant protein, E. coli culture was pelleted by centrifugation and resuspended in lysis buffer containing protease inhibitors. The cells were lysed using enzymatic lysis and sonication. The insoluble fraction of the lysate was subsequently separated from the soluble fraction using centrifugation and a sample of the soluble fraction was used to determine protein concentration by Lowry assay with a commercially available kit. The protein concentration was adjusted to 2 mg/ml and then an equal volume of 2X SDS-PAGE sample buffer was added.

Species of Origin :

E.coli

Species Reactivity :

Saccharomyces cerevisiae (Baker's yeast)

Stabilizer :

10% (v/v) Glycerol

Storage :

Store vial at -70° C or COLDER. For extended storage, aliquot contents to minimize freeze/thaw cycles.

Synonyms :

APG8, AUT7, CVT5, APG8 Positive Control Lysate

Target Species :

Saccharomyces cerevisiae

Background :

Ready-to-use positive control lysates produced by Rockland Immunochemicals are derived from bacterial strains, cell lines or tissues using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to the relevant epitope(s) that confirm the presence of the protein(s) of interest. This product is intended as a positive control for use with Rockland Immunochemicals anti-APG8 antibodies.

Epitope Tag Type :

FLAG

Low Endotoxin :

Lysate Fractionation :

Whole Cell Lysate

Lysate Stimulation :

Not Stimulated

MoBio Enzymes vs DNA vs MW Markers :

Other

MoBio Solution vs Reagent :

Reagent

PSC Type :

Protein

Sample Size :

Application Text :

Western Blot,

Category :

Controls

Conjugation Name :

Unconjugated

more info or order :

Rockland Immunochemicals product webpage