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company name :
Rockland Immunochemicals
product type :
other
product name :
STREPTAVIDIN PEROXIDASE Conjugated
catalog :
S000-03
quantity :
1
price :
184.00 USD
more info or order :
citations: 2
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product information
Catalog Number :
S000-03
Name :
STREPTAVIDIN PEROXIDASE Conjugated - S000-03
Display Name :
STREPTAVIDIN PEROXIDASE Conjugated
Application Note :
Streptavidin Peroxidase is a useful detection reagent for primary antibodies conjugated to biotin. Streptavidin Peroxidase can be utilized in Immunohistochemistry, Immunofluorescence, immuno-EM, Western Blotting, and ELISA experiment formats in combination with the proper substrate (TMB-1000 or FEMTOMAX-110).
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration Value :
1.0 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
HRP
Size :
1
Default Unit :
mg
ELISA Dilution :
1:20,000 - 1:200,000
Immunohistochemistry Dilution :
1:1,000 - 1:5,000
IF Microscopy Dilution :
User Optimized
Western Blot Dilution :
1:10,000 - 1:40,000
Expiration :
Expiration date is one (1) year from date of opening.
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Packing Type :
Ambient
Physical State :
Lyophilized
Preservative :
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Purity and Specificity :
Streptavidin-HRP was prepared from chromatographically purified streptavidin. Streptavidin Peroxidase conjugate was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Streptavidin.
Reconstitution Buffer :
Restore with deionized water (or equivalent)
Reconstitution Volume :
1.0 mL
Stabilizer :
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Storage :
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Streptavidin Peroxidase conjugated is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Synonyms :
HRP-SA, Horseradish Peroxidase conjugated S avidin, Streptococcus avidin conjugated to peroxidase, Streptavidin HRP, Streptavidin conjugated to horseradish peroxidase (HRP), HRP-linked Streptavidin
Background :
Streptavidin is isolated from bacteria, Streptomyces avidinii, and has an exceptionally high binding affinity for B7 (biotin). Rockland offers streptavidin in unconjugated and conjugated forms for common immunoassays including ELISA, western blotting, immunohistochemistry. Streptavidin is a tetrameric protein capable of binding 4 biotin groups to each molecule of streptavidin. While streptavidin has identical binding properties as avidin, it lacks the glycoprotein portion of the molecule and therefore shows less non-specific binding. Streptavidin is a slightly smaller molecule with a molecular weight of approximately 53.6 kDa. The sequence of avidin only shows 30% homology with streptavidin, and anti-avidin and anti-streptavidin antibodies are not immunologically cross reactive. Horseradish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs).
ABS Reagent Type :
Streptavidin
Low Endotoxin :
No
Sample Size :
No
Application Text :
ELISA,Immunohistochemistry,IF Microscopy,Western Blot,
Other :
User Optimized
Category :
Avidin, Biotin, Streptavidin
Conjugation Name :
Peroxidase (Horseradish)
2022 Price :
184.00 USD
UniProt :
P22629
NCBI :
CAA00084.1
Primary Image Name :
Peroxidase Conjugated Streptavidin - Western Blot
more info or order :
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
Pottstown, PA 19464
Info@rockland-inc.com
https://rockland-inc.com484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.