product summary
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company name :
Rockland Immunochemicals
product type :
other
product name :
SEPHAROSE™ PROTEIN A/G
catalog :
PAG50-00-0002
quantity :
2 mL
price :
83.00 USD
more info or order :
product information
Catalog Number :
PAG50-00-0002
Name :
SEPHAROSE™ PROTEIN A/G - PAG50-00-0002
Display Name :
SEPHAROSE™ PROTEIN A/G
Application Note :
Protein A/G resin may also be used to pull down antibody:antigen complexes in immunoprecipitation experiments. This product is suitable for use at 20 µL per immunoprecipitation reaction.
Buffer :
20% (v/v) Ethanol
Conjugation :
SEPH
Size :
2 mL
Default Unit :
mL
Immunoprecipitation Dilution :
User optimized
Western Blot Dilution :
User Optimized
Expiration :
Expiration date is one (1) year from date of opening.
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Other Performance Data :
The conjugate is provided as a suspension with 0.5 ml sepharose per 1 ml of suspension and is blocked with BSA to reduce non-specific immunoglobulin binding.
Packing Type :
Wet Ice
Physical State :
Suspension of agarose beads
Purity and Specificity :
The product can be used for binding IgG from human, rabbit, goat, mouse and rat. It has strong binding of human, rabbit, cow, sheep, and goat polyclonal antibodies, mouse IgG2a, IgG2b, IgG3, and rat IgG2a. Protein A/G also has moderate affinity for mouse and rat IgG1 and IgG2c. It binds with weak affinity to rat IgG2b. Sepharose™ Protein A/G can be used for immunoprecipitation and purification of monoclonal antibodies.
Storage :
Store SEPHAROSE PROTEIN A/G at 4° C prior to opening. DO NOT FREEZE.
Synonyms :
Sepharose Protein A/G, Protein AG, agarose, ProA-ProG, Staphylococcus A protein, Sepharose Protein A, Streptococcus G protein, Sepharose Protein G, immunoprecipitation, IP western blot
Background :
Sepharose™ Protein A/G can be used for immunoprecipitation and purification of monoclonal antibodies. Sepharose™ Protein A/G is a suspension of sepharose beads containing binding domains from both protein A (from Staphylococcus aureaus) and protein G (from Streptococcus sp.). When evenly dispersed approximately 2.0 ml of suspension is equal to 0.5 cc of settled beads of Sepharose™ 4 Fast Flow (highly cross linked, 4% agarose derivative). The bead size is approximately 45-165 µm when swollen. Each cc of drained beads will bind approximately 20-30 mg Human IgG. The exclusion limit (Mr) of the beads is 3 x 10^7. The maximum linear flow rate is approximately 1300 cm/hr. This product shows long term stability within pH range of 3 to 9 and short term stability within pH range of 2 to 10. Up to 100 reactions.
Low Endotoxin :
No
PSC Format :
Agarose Conjugate
Sample Size :
No
Application Text :
Immunoprecipitation,Western Blot,
Other :
User Optimized
Category :
Avidin, Biotin, Streptavidin
Conjugation Name :
Sepharose™
2020 Price :
83.00 USD
UniProt :
P19909
Primary Image Name :
SEPHAROSE™ Protein A/G - Bottle
more info or order :
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
Pottstown, PA 19464
Info@rockland-inc.com
https://rockland-inc.com484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.