product summary
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company name :
Rockland Immunochemicals
product type :
other
product name :
Protein A DyLight™ 488 Conjugated
catalog :
PA00-41
quantity :
100 µg
price :
98.00 USD
more info or order :
product information
Catalog Number :
PA00-41
Name :
PROTEIN A DyLight™ 488 Conjugated - PA00-41
Display Name :
Protein A DyLight™ 488 Conjugated
Application Note :
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration Value :
1.0 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
DL488
Size :
100 µg
Default Unit :
µg
FLISA Dilution :
>1:20,000
Fluorochrome Protein Value :
3.6
IF Microscopy Dilution :
>1:5,000
Western Blot Dilution :
>1:10,000
Expiration :
Expiration date is one (1) year from date of opening.
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Packing Type :
Ambient
Physical State :
Lyophilized
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
This product is chromatographically pure Protein A and shows predominantly a single band by SDS-PAGE. Greater than 95% of the A280 material binds to Human IgG.
Reconstitution Buffer :
Restore with deionized water (or equivalent)
Reconstitution Volume :
100 µL
Stabilizer :
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Storage :
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Synonyms :
Protein A DyLight™ 488 Conjugated, ProA, Staphylococcus A protein
Background :
Protein A is a 56 kDa cell wall protein originated from Staphylococcus aureus. It contains five homologous Ig-binding domains. Each domain is able to bind the Fc fragment of a wide range of mammalian immunoglobulins. Protein A affinity to Fc may vary in dependence of IgG species origins or subclasses. Protein A is often coupled to solid matrixes such as Sepharose™, magnetic particles or latex for IgG purification from ascites, serum or hybridoma culture media. Protein A is often coupled with reporter molecules such as a fluorescent dye, DyLight™, enzymes, biotin, or other detection agents.
ABS Reagent Type :
protein A
Low Endotoxin :
No
Sample Size :
No
Application Text :
FLISA,IF Microscopy,Western Blot,
Other :
User Optimized
Category :
Avidin, Biotin, Streptavidin
Conjugation Name :
DyLight™ 488
2020 Price :
98.00 USD
UniProt :
P38507
Primary Image Name :
Anti-Histone and Anti-tubulin Antibodies - Immunofluorescence Micrscopy
more info or order :
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
Pottstown, PA 19464
Info@rockland-inc.com
https://rockland-inc.com484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.