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company name :
Rockland Immunochemicals
product type :
other
product name :
BCIP/NBT Membrane Alkaline Phosphatase Substrate
catalog :
NBT-100
quantity :
100
price :
109.00 USD
more info or order :
product information
Catalog Number :
NBT-100
Name :
BCIP/NBT MEMBRANE ALKALINE PHOSPHATASE SUBSTRATE - NBT-100
Display Name :
BCIP/NBT Membrane Alkaline Phosphatase Substrate
Application Note :
BCIP/NBT Alkaline Phosphatase Membrane Substrate is a useful detection reagent for immunoblotting. The substrate is ready to use. To use: Wash the membrane after incubation with the alkaline phosphatase conjugate secondary reagent. Apply sufficient substrate to cover the membrane. Color (purple-turquoise, varies with pH) will develop within 30 minutes. Wash, dry membrane and image.
Concentration Value :
1X
Conjugation :
(None)
Size :
100
Default Unit :
mL
Immunohistochemistry Dilution :
1X
Western Blot Dilution :
1X
Expiration :
Expiration date is one (1) year from date of opening.
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Packing Type :
Ambient
Physical State :
Liquid - clear to pale yellow
Purity and Specificity :
pH: 9.28 +/- 0.1
QC Raw Material: PASS
Absorbance check of final product: PASS
Performance Data per Immunoblot: PASS
Stabilizer :
Proprietary buffer with enhancer
Storage :
Store container at 4° C prior to opening. Protect BCIP/NBT Membrane Alkaline Phosphatase Substrate from moisture and light. No special shipping conditions or precautions are required.
Synonyms :
5-Bromo-4-chloro-3-indonyl phosphate, X-phosphate, XP, Nitro-blue-tetrazolium chloride, Alkaline Phosphatase Substrate for Membranes, Alkaline Phosphatase Western Blot Substrate
Background :
BCIP/NBT Membrane Alkaline Phosphatase Substrate is an artificially manufactured chromogenic substrate for use in sensitive colorimetric assays. Alkaline phosphatase hydrolyzes the BCIP to 5-bromo-4-chloro-3-indole, which is oxidized by oxygen in the atmosphere, yielding a blue dye. In the presence of NBT, an insoluble dark blue precipitate is formed after a reduction reaction instead.
Low Endotoxin :
No
Sample Size :
No
Substrate Enzyme Type :
Alkaline Phosphatase
Substrate or Blocking :
Substrate
Application Text :
Immunohistochemistry,Western Blot,
Category :
Supporting Reagents
Conjugation Name :
Unconjugated
2022 Price :
109.00 USD
more info or order :
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
Pottstown, PA 19464
Info@rockland-inc.com
https://rockland-inc.com484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.