other

12 Epitope MBP Tag Protein Marker Lysate

MB-301-0100

100 µL

12 Epitope MBP Tag Protein Marker Lysate - MB-301-0100

12 Epitope MBP Tag Protein Marker Lysate

This 12 Epitope Tag Protein Marker Lysate is designed for use as a positive control for anti-epitope tag antibodies. This E.coli lysate contains a recombinant protein comprised of the following commonly used epitope tags: MBP--T7--HSV--cMyc--VSV--Glu-Glu--V5--E-tag--Flag--S tag--HA--6XHis. A 58 kDa recombinant protein is a visualized after SDS-polyacrylamide gel electrophoresis and western blotting using an antibody recognizing any of the expressed epitope tags listed above. The E.coli lysate was prepared by lysing cells in loading buffer/RIPA/Halt protease, then heating to 95° C for 10' followed by centrifugation at 13,000 g for 15'. Thaw the lysate to room temperature. Gently invert vial several times to ensure that the solution is homogeneous and that any precipitated material is re-dissolved. A loading volume of 0.25 to 2 µL is recommended for western blotting. This product is diluted in 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCL, 2% SDS, 10% Glycerol and 0.005% Bromophenol Blue, pH 6.8).

See application note.

5.0 mg/mL

by modified Lowry assay

(None)

100 µL

ul

User Optimized

Expiration date is one (1) year from date of opening.

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Dry Ice

Liquid (in 1x Loading Buffer)

Loading control for Western blotting. Lysate Marker expresses 12 different epitope tags.

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing.

Flag-tag marker, MBP-tag marker, HA-tag marker, 6XHIS-tag marker, T7-tag marker, cMyc-tag marker, V5-tag marker, S-tag marker, HSV-tag marker, VSV-tag marker, Glu-Glu-tag marker, E-tag marker, Lysate marker, Protein Marker, Loading control, epitope tag marker, positive control marker

Epitope tags are short peptide sequences easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties.   Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins.  This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.

No

MW Standards

Reagent

No

Western Blot,

User Optimized

Molecular Biology Reagents

Unconjugated

12-Epitope Tag Protein Marker Lysate - Western Blot