product summary
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company name :
Rockland Immunochemicals
product type :
other
product name :
Nuclease S1
catalog :
MB-110-0010
quantity :
10 ku
more info or order :
product information
Catalog Number :
MB-110-0010
Name :
Nuclease S1 (Aspergillus oryzae sp.) - MB-110-0010
Display Name :
Nuclease S1
Application Note :
One unit renders acid soluble 1 µg of denatured DNA (ssDNA) per minute at 37° C, pH 4.6. This assay is based on the release of acid soluble products from ssDNA through hydrolysis. One (1) µg of acid soluble products is equivalent to 0.022 A260 units. A solution in 30 mM Sodium Acetate, 50 mM Sodium Chloride, 1 mM Zinc Chloride and 50% (v/v) glycerol, pH 4.6.
Buffer :
See application note.
Conjugation :
(None)
Size :
10 ku
Default Unit :
ku
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Packing Type :
Dry Ice
Physical State :
Liquid (sterile filtered)
Specific Activity Value :
222,990 units/mL
Specific Activity Definition :
by dry weight
Storage :
Store vial at -20° C or colder. Expiration date is six (6) months from date of opening vial.
Synonyms :
S1 Nuclease
Background :
S1 nuclease is an endonuclease that is active against single-stranded DNA and RNA molecules. It is five times more active on DNA than RNA. Although its primary substrate is single-stranded, it can also occasionally introduce single-stranded breaks in double-stranded DNA or RNA, or DNA-RNA hybrids. S1 nuclease is used in the laboratory as a reagent in nuclease protection assays. In molecular biology, it is used in removing single stranded tails from DNA molecules to create blunt ended molecules and opening hairpin loops generated during synthesis of double stranded cDNA.
Low Endotoxin :
No
MoBio Enzymes vs DNA vs MW Markers :
Enzyme
MoBio Solution vs Reagent :
Reagent
Sample Size :
No
Other :
User Optimized
Category :
Molecular Biology Reagents
Conjugation Name :
Unconjugated
more info or order :
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.