product summary
Loading...
company name :
Rockland Immunochemicals
product type :
other
product name :
Cell Lysis Buffer
catalog :
MB-078-0015
quantity :
15 mL
more info or order :
product information
Catalog Number :
MB-078-0015
Name :
Cell Lysis Buffer - MB-078-0015
Display Name :
Cell Lysis Buffer
Application Note :
The buffer is ready to use for all types of human or animal cells. Lysed cells can be used for Western Blot, co-Immunoprecipitation, Chromatin IP (ChIP), enzyme activity assays, and various other cell based assays.
Buffer :
See application note.
Concentration Value :
1X
Size :
15 mL
Default Unit :
mL
ChIP Dilution :
User Optimized
Immunoprecipitation Dilution :
User Optimized
Western Blot Dilution :
User Optimized
Expiration :
This product MAY be stable for up to two (2) weeks if properly stored and handled.
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Packing Type :
Ambient
Physical State :
Liquid (sterile filtered)
Purity and Specificity :
This product was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
Sterilization :
Sterile Aseptic Filter
Storage :
Store cell lysis buffer at -20° C prior to opening. Buffer is stable for up to 2 years stored at -20°C. It is recommended to aliquot and freeze buffer for extended usage. Store thawed buffer at 2-8°C for no longer than 2 weeks.
Synonyms :
Cell Lysis Buffer
Background :
Cell Lysis Buffer is used to lyse cells under non-denaturing conditions. If recommended add 1 mM PMSF or other protease inhibitor immediately before use. Keep all reagents and lysates in ice.
For adherent cells add 400µL of buffer per 10cm flask once they have been washed in 1X PBS.
For suspended cells add 400 µL of buffer per million cells once they have been washed in 1X PBS and pelleted.
For tissue lysates add 20µL of buffer per 1mg of tissue followed by homogenization and sonication.
Low Endotoxin :
No
MoBio Solution vs Reagent :
Solution
MoBio Water vs Buffer :
Buffer
Sample Size :
No
Application Text :
Chip,Immunoprecipitation,Western Blot,
Other :
User Optimized
Category :
Molecular Biology Reagents
more info or order :
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.