product summary
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company name :
Rockland Immunochemicals
product type :
other
product name :
Ponceau S solution
catalog :
MB-072-0500
quantity :
500 mL
more info or order :
Rockland Immunochemicals product webpage
product information
Catalog Number :
MB-072-0500
Name :
Ponceau S solution - MB-072-0500
Display Name :
Ponceau S solution
Application Note :
Store at room temperature. The product is at working concentration, no further dilution is necessary. The limit of detection for this stain is 200 nanograms of protein after separation by electrophoresis in polyacrylamide gels and transferred to nitrocellulose or PVDF membranes. Ponceau S is not suitable for use with nylon membranes.
Concentration Value :
1X
Conjugation :
(None)
Size :
500 mL
Default Unit :
mL
Western Blot Dilution :
ready to use
Expiration :
Expiration date is six (6) months from date of opening.
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Packing Type :
Ambient
Physical State :
Liquid
Purity and Specificity :
Ponceau S Staining Solution contains Ponceau S in a specially formulated acetate buffer for optimal staining results. Ponceau S is a ready-to-use stain that can be used to quickly and reversible stain of proteins on blotting membranes after Western blot transfer. Staining of Nylon membranes is not recommended.
Storage :
Store container at room temperature (20° to 26° C).
Synonyms :
Staining, Western Blot membrane stains, PVDF stains, nitrocellulose stain, reversible membrane stain, cellulose acetate membrane stain
Background :
Ponceau S Staining Solution is made using Ponceau S. Ponceau S is a sodium salt used for the detection of proteins on PVDF, and nitrocellulose membranes. Staining of blot transfer membranes permits visualization of proteins and allows the extent of transfer to be monitored. Ponceau S binds rapidly and reversibly to the positively charged amino groups and binds non-covalently to non-polar regions in the protein. Ponceau S stain is designed for rapid (5 min) staining on nitrocellulose or PVDF membranes and also for staining protein on cellulose acetate membranes. Microgram quantities of transferred protein will be detected as red protein bands with a clear background. Ponceau S staining on nitrocellulose and PVDF is reversible by washing the stained membrane with dH2O or blocking buffer, this allows further immunological detection.
Low Endotoxin :
No
MoBio Solution vs Reagent :
Solution
MoBio Water vs Buffer :
Buffer
Sample Size :
No
Application Text :
Western Blot,
Category :
Molecular Biology Reagents
Conjugation Name :
Unconjugated
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.