AKT1 Western Chemiluminescent Blotting Kit | Rockland Immunochemicals KCAI51 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

AKT1 Western Chemiluminescent Blotting Kit

catalog :

KCAI51

quantity :

1 Kit

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

14E5.A2.B2.H9

reactivity :

human

application :

western blot

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

KCAI51

Name :

AKT1 Western Blot Kit - KCAI51

Display Name :

AKT1 Western Chemiluminescent Blotting Kit

Application Note :

Use Rockland Immunochemicals’ Anti-AKT1 Chemiluminescent Kit for Western Blotting for detection of human AKT1 native and recombinant proteins by western blot. Expect a band approximately 56 kDa in size corresponding to AKT1 protein. This kit is useful for both western blotting and dot blotting methods. Please read the entire product insert prior to use.

Clonality :

Monoclonal

Clone ID :

14E5.A2.B2.H9

Conjugation :

(None)

Size :

1 Kit

Default Unit :

Kit

Western Blot Dilution :

User Optimized

Expiration :

See kit insert for complete instructions.

Format :

IgG2a

Gene Name :

AKT1

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Packing Type :

Dry Ice

Preservative :

Wash buffers MUST NOT contain SODIUM AZIDE or other inhibitors of peroxidase activity!

Purity and Specificity :

The kit is designed to detect both unphosphorylated and phosphorylated forms of the protein. Cross reactivity with AKT1 from other species has not been determined, however, the sequence of the immunogen shows 100% identity to human, mouse, and rat, therefore, cross reactivity is expected. Cross-reactivity with AKT2 and AKT3 has not been determined. This kit contains sufficient substrate for up to 5 mini blots at 7.5 x 8 cm2 (1,800 cm2) and is stable for at least 1 year when stored as indicated.

Species Reactivity :

human

Storage :

See kit insert for complete instructions.

Subclass :

IgG2a kappa

Synonyms :

AKT 1 western blotting, AKT-1, PKB antibody, PKB gamma antibody, PKBGAMMA antibody, PRKBG antibody, Protein kinase Akt 1 antibody, Protein kinase B gamma antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma, Chemiluminescent WB, Western Blotting Kit, Chemiluminescent Kit, Peroxidase Kit, Immunoblotting Kit

Target Species :

Human

Background :

Rockland Immunochemicals’ Chemiluminescent Western Blot Kit for AKT1 combines all of the necessary reagents with a rapid proven protocol and the extremely high signal detection of our luminol chemiluminescent substrate for the detection of native and/or recombinant human AKT1 protein. AKT1 Western Bloting kit allows for the detection of endogenous protein levels of human AKT1 present in cell lysates provided by the user. As a positive control, this kit includes a MDA-MB468 whole cell lysate proven to contain AKT1. After protein separation by SDS-PAGE and transfer, the membrane is probed with Rockland's optimized Anti-AKT1 antibody. Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. AKT1 Western Blotting is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience and Signal Transduction research.

Detection Kit Type :

Chemiluminescent Western Blot Kit

Detection Kit vs Component :

Kit

Low Endotoxin :

Sample Size :

Application Text :

Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P31749

Primary Image Name :

Monoclonal mouse anti AKT1 - WB - Cell lines

more info or order :

Rockland Immunochemicals product webpage