product summary
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company name :
Rockland Immunochemicals
product type :
other
product name :
Bovine Serum Albumin - Fraction V
catalog :
BSA-50
quantity :
50
price :
113.00 USD
more info or order :
Rockland Immunochemicals product webpage
citations: 11
Reference
Schuster Little N, Madera S, Whelan R. Developing a mass spectrometry-based assay for the ovarian cancer biomarker CA125 (MUC16) using suspension trapping (STrap). Anal Bioanal Chem. 2020;: pubmed publisher
Pollock S, Schinlever A, Rohani A, Kashatus J, Kashatus D. RalA and RalB relocalization to depolarized mitochondria depends on clathrin-mediated endocytosis and facilitates TBK1 activation. PLoS ONE. 2019;14:e0214764 pubmed publisher
Zhang J, He J, Johnson J, Napolitano G, Ramadass M, Rahman F, et al. Cross-regulation of defective endolysosome trafficking and enhanced autophagy through TFEB in UNC13D deficiency. Autophagy. 2019;:1-19 pubmed publisher
Clausen T, Christoffersen S, Dahlbäck M, Langkilde A, Jensen K, Resende M, et al. Structural and functional insight into how the Plasmodium falciparum VAR2CSA protein mediates binding to chondroitin sulfate A in placental malaria. J Biol Chem. 2012;287:23332-45 pubmed publisher
Dahlbäck M, Jørgensen L, Nielsen M, Clausen T, Ditlev S, Resende M, et al. The chondroitin sulfate A-binding site of the VAR2CSA protein involves multiple N-terminal domains. J Biol Chem. 2011;286:15908-17 pubmed publisher
Lee C, Bose S, Van Vliet K, Karp J, Karnik R. Studying cell rolling trajectories on asymmetric receptor patterns. J Vis Exp. 2011;: pubmed publisher
Makino N, Maeda T, Oyama J, Sasaki M, Higuchi Y, Mimori K, et al. Antioxidant therapy attenuates myocardial telomerase activity reduction in superoxide dismutase-deficient mice. J Mol Cell Cardiol. 2011;50:670-7 pubmed publisher
Moorjani S, Nielson R, Chang X, Shear J. Dynamic remodeling of subcellular chemical gradients using a multi-directional flow device. Lab Chip. 2010;10:2139-46 pubmed publisher
Resende M, Ditlev S, Nielsen M, Bodevin S, Bruun S, Pinto V, et al. Chondroitin sulphate A (CSA)-binding of single recombinant Duffy-binding-like domains is not restricted to Plasmodium falciparum Erythrocyte Membrane Protein 1 expressed by CSA-binding parasites. Int J Parasitol. 2009;39:1195-204 pubmed publisher
Dravid G, Hammond H, Cheng L. Culture of human embryonic stem cells on human and mouse feeder cells. Methods Mol Biol. 2006;331:91-104 pubmed
De la Maza M, Olivares D, Hirsch S, Sierralta W, Gattas V, Barrera G, et al. Weight increase and overweight are associated with DNA oxidative damage in skeletal muscle. Clin Nutr. 2006;25:968-76 pubmed
product information
Catalog Number :
BSA-50
Name :
BOVINE SERUM ALBUMIN - Fraction V (Immunoglobulin and Protease Free) - BSA-50
Display Name :
Bovine Serum Albumin - Fraction V
Application Note :
Bovine Serum Albumin - Fraction V is suitable for use in protease sensitive assays such as RIA, EIA and nucleic acid hybridization, use as a stabilizing agent for proteins and enzymes including dilute solutions of antibody, use as a blocking agent to reduce non-specific binding for FACS, IF, IHC, and WB.
Conjugation :
(None)
Size :
50
Default Unit :
g
ELISA Dilution :
Yes
FLISA Dilution :
Yes
Flow Cytometry Dilution :
Yes
Immunohistochemistry Dilution :
Yes
IF Microscopy Dilution :
Yes
Immunoprecipitation Dilution :
Yes
Western Blot Dilution :
Yes
Expiration :
Expiration date is one (1) year from date of opening.
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Other Performance Data :
Moisture (%) 1.0 % Loss on Drying pH: 7.0 Protein (%): 99.7 % by nitrogen analysis. Protease: <0.005 Units/mg Heavy Metals: < 0.46 ppm by ICP Endotoxin: < 0.1 EU/mg by LAL
Packing Type :
Ambient
Physical State :
Lyophilized
Purity and Specificity :
Purity (%): 100% by Agarose Zone Electrophoresis Moisture (%): <0.9% pH: 7.0 Protein (%): 100.5% by nitrogen analysis. Sodium Chloride (mg/g): <5.0 by titration Protease: <0.005 units/mg by Enzymatic Assay. Immunoglobulin: None Detected by Radial Immunodiffusion. Heavy Metals: <0.49 ppm
Reconstitution Buffer :
Restore with deionized water (or equivalent)
Storage :
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Synonyms :
BSA Blocker, BSA Blocking, FACS Buffer
Background :
Bovine Serum Albumin (BSA) is used for various biochemical applications including ELISA (Enzyme-Linked Immunosorbent Assay), high content screenining assays, western blotting, FACS Buffer and immunohistochemistry. BSA as a blocking reagent is particularly useful with casein-sensitive antibodies, such as phospho-specific antibodies. Also used as a nutrient in cell and microbial culture. In restriction digests, BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels. Bovine Serum Albumin can also be used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA.
Blocker Type :
BSA
Low Endotoxin :
No
Sample Size :
No
Substrate or Blocking :
Blocking Reagent
Application Text :
ELISA,FLISA,Flow Cytometry,Immunohistochemistry,IF Microscopy,Immunoprecipitation,Western Blot,
Category :
Supporting Reagents
Conjugation Name :
Unconjugated
2022 Price :
113.00 USD
Primary Image Name :
BOVINE SERUM ALBUMIN
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
Info@rockland-inc.com
https://rockland-inc.com
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.