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company name :
Rockland Immunochemicals
product type :
other
product name :
Blotto Immunoanalytical Grade (Non-Fat Dry Milk)
catalog :
B501-0500
quantity :
500
price :
81.00 USD
more info or order :
product information
Catalog Number :
B501-0500
Name :
BLOTTO Immunoanalytical Grade (Non-Fat Dry Milk) - B501-0500
Display Name :
Blotto Immunoanalytical Grade (Non-Fat Dry Milk)
Application Note :
Use Rockland's BLOTTO whenever procedures call for immunoanalytical grade non-fat dry milk. Alternative blocking agents such as BLOTTO A (solution of TBS, 5% non-fat dry milk and 0.05% Tween-20) and BLOTTO B (solution of TBS, 1% non-fat dry milk, 1% BSA and 0.05% Tween-20) are also available. BLOTTO A is a convenient general purpose blocking agent. BLOTTO B is recommended for blocking when phospho-specific antibodies are used. BLOTTO A and BLOTTO B provide Tween-20 in a separate vial in liquid form (p/n TW0025). Users should consider the addition of protease inhibitors and phosphatase inhibitors when required. Typically 50 mM NaF can be added to BLOTTO solutions to inhibit phosphatase activity.
Conjugation :
(None)
Size :
500
Default Unit :
g
ELISA Dilution :
User Optimized
IF Microscopy Dilution :
User Optimized
Western Blot Dilution :
User Optimized
Expiration :
Expiration date is one (1) year from date of opening.
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Packing Type :
Ambient
Physical State :
Lyophilized
Purity and Specificity :
This product contains highly purified immunoanalytical grade non-fat dry milk.
Reconstitution Buffer :
Restore with deionized water (or equivalent)
Storage :
Store container at room temperature prior to opening. After reconstitution, use blocking buffers immediately. Dilute solutions may be stored at 4° C for up to four (4) days. Solutions containing BLOTTO may be frozen.
Synonyms :
Blotto Immunoanalytical Grade Non-Fat Dry Milk, Blocking reagent for western, antibody dilution buffer
Background :
Rockland Immunochemicals produces the following blocking buffer reagents: BSA, BLOTTO, BLOTTO A, BLOTTO B and Blocking Buffer for Fluorescent Western Blotting (see related products below). These products are intended to block non-specific binding of proteins in various immunological assays. Typically, western blotting and ELISA methods call for the addition of a blocking agent prior to the addition of primary antibody.
Blocker Type :
BLOTTO
Low Endotoxin :
No
Sample Size :
No
Substrate or Blocking :
Blocking Reagent
Application Text :
ELISA,IF Microscopy,Western Blot,
Other :
User Optimized
Category :
Supporting Reagents
Conjugation Name :
Unconjugated
2022 Price :
81.00 USD
Primary Image Name :
Blotto Immunoanalytical Grade Bottles
more info or order :
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
Pottstown, PA 19464
Info@rockland-inc.com
https://rockland-inc.com484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.