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company name :
Rockland Immunochemicals
product type :
other
product name :
Biotin Beta Galactosidase Conjugated
catalog :
B000-17
quantity :
1 mg
price :
175.00 USD
more info or order :
product information
Catalog Number :
B000-17
Name :
BIOTIN BETA GALACTOSIDASE Conjugated - B000-17
Display Name :
Biotin Beta Galactosidase Conjugated
Application Note :
Biotin Beta Galactosidase Conjugated has been assayed against 1.0 ug of Streptavidin in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 is suggested for Biotin Beta Galactosidase Conjugated.
Buffer :
0.05 M Potassium Phosphate, 0.001M Magnesium Chloride, 0.1M Beta-Mercaptoethanol; pH 7.8
Concentration Value :
1.0 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
BAC
Size :
1 mg
Default Unit :
mg
ELISA Dilution :
1:8,000 - 1:32,000
Immunohistochemistry Dilution :
1:200 - 1:1,000
Western Blot Dilution :
1:500 - 1:2,500
Expiration :
Expiration date is one (1) year from date of opening.
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Packing Type :
Wet Ice
Physical State :
Liquid (sterile filtered)
Purity and Specificity :
Biotin Beta Galactosidase Conjugated was prepared from electrophoretically pure Beta Galactosidase and Biotin. Biotin Beta Galactosidase assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Beta Galactosidase (E.coli) and anti-Biotin.
Storage :
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing Beta Galactosidase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.
Synonyms :
Vitamin H, Coenzyme R, Vitamin B7, β-galactosidase, β-gal, beta-gal, Biotin Beta Galactosidase Conjugated, Biotin Beta Gal Conjugated
Background :
Biotin is a small biomolecule important for many cellular processes. Most importantly for biotechnology applications, biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptavidin; an attraction that is the strongest and most stable non-covalent interaction known.
Beta-galactosidase (beta-gal; β-gal) is an enzyme that hydrolyzes β-galactosides (such as lactose and various glycoproteins) into simple sugars. Beta-galactosidase is routinely used as a reporter in biochemical experiments as well as a marker for gene expression experiments in E. coli. Beta-galactosidase can be used to cleave the chemical X-gal that forms an intense blue product that can easily be quantified.
ABS Reagent Type :
Biotin
Low Endotoxin :
No
Sample Size :
No
Application Text :
ELISA,Immunohistochemistry,Western Blot,
Other :
User Optimized
Category :
Avidin, Biotin, Streptavidin
Conjugation Name :
Biotin
2020 Price :
175.00 USD
Primary Image Name :
Biotin Beta Galactosidase Conjugated Dot Blot
more info or order :
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
Pottstown, PA 19464
Info@rockland-inc.com
https://rockland-inc.com484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.