NAG-1 Antibody
Rockland Immunochemicals
catalog: 600-401-B07

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry

Immunohistochemistry of Rabbit anti NAG1 antibody (C-terminal specific) at 20X in colon tissue at pH 9 . Negative control of human colon tissue pH9 is shown in background. Tissue: Human Colon
quantity: 100
price: 449.00 USD
to the supplier

NAG-1 Antibody
Rockland Immunochemicals
catalog: 600-401-B08

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

In this sandwich ELISA, NAG-1 was captured from human serum using the following antibodies (see Related Products below): anti-NAG-1/GDF15 (C terminal specific), anti-NAG-1/GDF15 (N terminal specific (PAN)), anti-NAG-1/GDF15 (H-variant) and anti-NAG-1/GDF15 (D-variant) polyclonal antibodies. Micro titer plates were coated with capture antibody at 1 µg/mL. Control plates received PBS only (data not shown). After overnight incubation and blocking, independent experiments using 20 random normal human sera were performed. Neat normal sera were applied and incubated for 1 h at 37 °C. After washing, HRP conjugated anti-NAG-1/GDF15 (C terminal specific) antibody was added for detection at 100 µL per well at 1 µg/mL. Following further incubation for 1 hr at 37°C, the plates were washed and TMBE was added as an HRP substrate for 30 min. The reaction was stopped by 1 M H2SO4 and values were measured at 450nm.
quantity: 100 µg
price:
to the supplier

NAG-1 Antibody
Rockland Immunochemicals
catalog: 600-401-B09

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

In this sandwich ELISA, NAG-1 was captured from human serum using the following antibodies (see Related Products below): anti-NAG-1/GDF15 (C terminal specific), anti-NAG-1/GDF15 (N terminal specific (PAN)), anti-NAG-1/GDF15 (H-variant) and anti-NAG-1/GDF15 (D-variant) polyclonal antibodies. Micro titer plates were coated with capture antibody at 1 µg/mL. Control plates received PBS only (data not shown). After overnight incubation and blocking, independent experiments using 20 random normal human sera were performed. Neat normal sera were applied and incubated for 1 h at 37 °C. After washing, HRP conjugated anti-NAG-1/GDF15 (C terminal specific) antibody was added for detection at 100 µL per well at 1 µg/mL. Following further incubation for 1 hr at 37°C, the plates were washed and TMBE was added as an HRP substrate for 30 min. The reaction was stopped by 1 M H2SO4 and values were measured at 450nm.
quantity: 100 µg
price:
to the supplier

NAG-1 Antibody
Rockland Immunochemicals
catalog: 600-401-B10

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot using Rockland's affinity purified anti-NAG-1/GDF15 (N-terminal specific) antibody shows detection of a 14 kDa band corresponding to recombinant human NAG-1 purified from CHO cells.   Samples were electrophoresed on a 4-20% gradient gel under reducing conditions.   Lane 1 shows NAG-1 detection.  Lane 2 shows reactivity is greatly diminished when this antibody is preincubated with the immunizing peptide prior to Western blotting.
quantity: 100 µg
price:
to the supplier

NAG-1 Antibody
Rockland Immunochemicals
catalog: 200-301-B07

mouse monoclonal (23B3.D2.H5)
reactivity: human
application: western blot, ELISA

Western blot using Rockland's anti-NAG-1 monoclonal antibody. The blot shows detection of recombinant NAG-1 protein present in Pichia pastoris whole cell lysates: lane 1 - yeast cell lysate expressing NAG-1 H variant with SUMO expression tag at 36 kDa; lane 2 - yeast cell lysate expressing NAG-1 D variant with SUMO expression tag at 36 kDa; lane 3 - yeast cell lysate expressing NAG-1 H variant; and lane 4 - yeast cell lysate expressing NAG-1 D variant. Recombinant NAG-1 proteins without SUMO correspond to monomer (15 kDa) and dimer (30 kDa) bands as indicated by the arrowheads. All lysates were run under reducing conditions. Primary antibody was used at a 1:1,000 dilution in TBS containg 1% BSA and 0.2% Tween, and reacted overnight at 4°C. For detection, a 1:40,000 dilution of peroxidase conjugated Gt-a-Mouse IgG secondary antibody (610-103-121) was used in Blocking Buffer for Fluorescent Western Blotting (MB-070) for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc™ 4000MP Imaging System.
quantity: 100 µg
price:
to the supplier

NAG-1 H Variant Antibody
Rockland Immunochemicals
catalog: 200-301-B08

mouse monoclonal (7C12.B3.F2)
reactivity: human
application: western blot, ELISA

Western blot shows detection of recombinant NAG-1 protein present in Pichia pastoris whole cell lysates: lane 1 - yeast cell lysate expressing NAG-1 H variant with SUMO expression tag at 36 kDa; lane 2 - yeast cell lysate expressing NAG-1 D variant with SUMO expression tag at 36 kDa; lane 3 - yeast cell lysate expressing NAG-1 H variant; and lane 4 - yeast cell lysate expressing NAG-1 D variant. All lysates were run under reducing conditions. Primary antibody was used at a 1:1,000 dilution in TBS containing 1% BSA and 0.2% Tween, and reacted overnight at 4°C. For detection, a 1:40,000 dilution of peroxidase conjugated Gt-a-Mouse IgG secondary antibody (610-103-121) was used in Blocking Buffer for Fluorescent Western Blotting (MB-070) for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc™ 4000MP Imaging System.
quantity: 100 µg
price:
to the supplier

NAG-1 Antibody
Rockland Immunochemicals
catalog: 200-301-B10

mouse monoclonal (23G10.E4.F8)
reactivity: human
application: western blot, ELISA

Western blot shows detection of recombinant NAG-1 protein (arrow) present in Pichia pastoris whole cell lysates: lane 1 - yeast cell lysate expressing NAG-1 H variant with SUMO expression tag at 36 kDa; lane 2 - yeast cell lysate expressing NAG-1 D variant with SUMO expression tag at 36 kDa; lane 3 - yeast cell lysate expressing NAG-1 H variant; and lane 4 - yeast cell lysate expressing NAG-1 D variant. All lysates were run under reducing conditions. Primary antibody was used at a 1:1000 dilution in TBS containing 1% BSA and 0.2% Tween, and reacted overnight at 4°C. For detection, a 1:40,000 dilution of peroxidase conjugated Gt-a-Mouse IgG secondary antibody (610-103-121) was used in Blocking Buffer for Fluorescent Western Blotting (MB-070) for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc™ 4000MP Imaging System.
quantity: 100 µg
price:
to the supplier

NAG-1 Antibody
Rockland Immunochemicals
catalog: 200-501-B07

rat monoclonal (6D12.H10.E4)
reactivity: human
application: western blot, ELISA

quantity: 100 µg
price:
to the supplier

NAG-1 Antibody
Rockland Immunochemicals
catalog: 209-401-B88

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA

Western blot using Rockland's affinity purified anti-Human NAG-1 antibody shows detection of a 42 kDa band corresponding to purified recombinant fusion protein MBP-human NAG-1.   Samples were electrophoresed on a 4-20% gradient gel under reducing conditions. Primary antibody was used at 1:1000 in 1% BSA-TBST overnight at 4°C. DyLight649™ Goat Anti-Rabbit IgG secondary antibody (p/n 611-143-122) was used at 1:20,000 in Blocking Buffer for Fluorescent Western Blotting (MB-070) for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc™ 4000MP Imaging System. Other detection systems will yield similar results.
quantity: 100 µg
price:
to the supplier

NAG-1 Antibody Peroxidase Conjugated
Rockland Immunochemicals
catalog: 209-403-B88

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: HRP
application: western blot, ELISA

quantity: 100 µg
price:
to the supplier

NAG-1 Antibody Biotin Conjugated
Rockland Immunochemicals
catalog: 209-406-B88

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

quantity: 100 µg
price:
to the supplier

NAG-1 Antibody
Rockland Immunochemicals
catalog: 210-401-B68

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA

Western blot using Rockland's affinity purified anti-Mouse NAG-1/GDF15 antibody. The blot shows detection of recombinant MBP-NAG-1 fusion protein (60 kDa) purified from E.coli (lane 1); yeast cell lysate expressing SUMO-mouse NAG-1 (42 kDa) (lane 2), and R&D human NAG-1 monomer purified from CHO-K1 cells (14 kDa) (lane 3). All lysates were run under reducing conditions. Primary antibody was used at a 1:1000 dilution in TBS containg 1% BSA and 0.2% Tween, and reacted overnight at 4°C. Nag-1 was detected using a 1:40,000 dilution of peroxidase conjugated Gt-a-Rabbit antibody (611-103-122) in Blocking Buffer for Fluorescent Western Blotting (MB-070) for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc™ 4000MP Imaging System. Other detection systems will yield similar results.
quantity: 100 µg
price:
to the supplier

NAG-1 Antibody Peroxidase Conjugated
Rockland Immunochemicals
catalog: 210-403-B68

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

quantity: 100 µg
price:
to the supplier

NAG-1 Antibody Biotin Conjugated
Rockland Immunochemicals
catalog: 210-406-B68

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

quantity: 100 µg
price:
to the supplier

NAG-1 Antibody Peroxidase Conjugated
Rockland Immunochemicals
catalog: 600-403-B07

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

Western blot using Rockland's affinity purified anti-NAG-1/GDF15 (C-terminal) antibody shows detection NAG-1 purified from CHO cells as a 14 kDa band corresponding to monomer and a 28 kDa band corresponding to dimerized NAG-1. Samples were electro-phoresed on a 4-20% gradient gel under reducing conditions. Lane 1 shows NAG-1 detection. Lane 2 shows reactivity is blocked when this antibody is pre-incubated with the immunizing peptide prior to Western blotting.
quantity: 100 µg
price:
to the supplier

NAG-1 Antibody Peroxidase Conjugated
Rockland Immunochemicals
catalog: 600-403-B08

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA

In this sandwich ELISA, NAG-1 was captured from human serum using the following antibodies (see Related Products below): anti-NAG-1/GDF15 (C terminal specific), anti-NAG-1/GDF15 (N terminal specific (PAN)), anti-NAG-1/GDF15 (H-variant) and anti-NAG-1/GDF15 (D-variant) polyclonal antibodies. Micro titer plates were coated with capture antibody at 1 µg/mL. Control plates received PBS only (data not shown). After overnight incubation and blocking, independent experiments using 20 random normal human sera were performed. Neat normal sera were applied and incubated for 1 h at 37 °C. After washing, HRP conjugated anti-NAG-1/GDF15 (C terminal specific) antibody was added for detection at 100 µL per well at 1 µg/mL. Following further incubation for 1 hr at 37°C, the plates were washed and TMBE was added as an HRP substrate for 30 min. The reaction was stopped by 1 M H2SO4 and values were measured at 450nm.
quantity: 100 µg
price:
to the supplier

NAG-1 Antibody Peroxidase Conjugated
Rockland Immunochemicals
catalog: 600-403-B09

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA

quantity: 100 µg
price:
to the supplier

NAG-1 Antibody Peroxidase Conjugated
Rockland Immunochemicals
catalog: 600-403-B10

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA

quantity: 100 µg
price:
to the supplier

NAG-1 Antibody Biotin Conjugated
Rockland Immunochemicals
catalog: 600-406-B07

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

Western blot using Rockland's affinity purified anti-NAG-1/GDF15 (C-terminal) antibody shows detection NAG-1 purified from CHO cells as a 14 kDa band corresponding to monomer and a 28 kDa band corresponding to dimerized NAG-1. Samples were electro-phoresed on a 4-20% gradient gel under reducing conditions. Lane 1 shows NAG-1 detection. Lane 2 shows reactivity is blocked when this antibody is pre-incubated with the immunizing peptide prior to Western blotting.
quantity: 100 µg
price:
to the supplier

NAG-1 Antibody Biotin Conjugated
Rockland Immunochemicals
catalog: 600-406-B08

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

In this sandwich ELISA, NAG-1 was captured from human serum using the following antibodies (see Related Products below): anti-NAG-1/GDF15 (C terminal specific), anti-NAG-1/GDF15 (N terminal specific (PAN)), anti-NAG-1/GDF15 (H-variant) and anti-NAG-1/GDF15 (D-variant) polyclonal antibodies. Micro titer plates were coated with capture antibody at 1 µg/mL. Control plates received PBS only (data not shown). After overnight incubation and blocking, independent experiments using 20 random normal human sera were performed. Neat normal sera were applied and incubated for 1 h at 37 °C. After washing, HRP conjugated anti-NAG-1/GDF15 (C terminal specific) antibody was added for detection at 100 µL per well at 1 µg/mL. Following further incubation for 1 hr at 37°C, the plates were washed and TMBE was added as an HRP substrate for 30 min. The reaction was stopped by 1 M H2SO4 and values were measured at 450nm.
quantity: 100 µg
price:
to the supplier

NAG-1 Antibody Biotin Conjugated
Rockland Immunochemicals
catalog: 600-406-B09

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

quantity: 100 µg
price:
to the supplier

NAG-1 Antibody Biotin Conjugated
Rockland Immunochemicals
catalog: 600-406-B10

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

quantity: 100 µg
price:
to the supplier

Nag-1 Antibody
Rockland Immunochemicals
catalog: 610-401-B87

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA

Western blot using Rockland's affinity purified anti-mouse NAG-1/GDF15 antibody. The blot shows detection of recombinant MBP-NAG-1 fusion protein (60 kDa) purified from E.coli (lane 1); yeast cell lysate expressing SUMO-mouse NAG-1 (42 kDa) (lane 2), and R&D human NAG-1 monomer purified from CHO-K1 cells (14 kDa) (lane 3). All lysates were run under reducing conditions. Primary antibody was used at a 1:1000 dilution in TBS containg 1% BSA and 0.2% Tween, and reacted overnight at 4°C. Nag-1 was detected using a 1:40,000 dilution of peroxidase conjugated Gt-a-Rabbit antibody (611-103-122) in Blocking Buffer for Fluorescent Western Blotting (MB-070) for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc™ 4000MP Imaging System. Other detection systems will yield similar results.
quantity: 100 µg
price:
to the supplier