Neurofilament M Antibody | Rockland Immunochemicals 912-301-D84 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Neurofilament M Antibody

catalog :

912-301-D84

quantity :

100 µL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3H11

reactivity :

mouse, rat

application :

western blot

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

912-301-D84

Name :

Anti-Neurofilament M (Mouse) Antibody - 912-301-D84

Display Name :

Neurofilament M Antibody

Application Note :

Anti-NFM antibody is suitable for use in Western Blotting and IF. Expect a band of approximately 145 kDa in size corresponding to the NF-M proteins in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

3H11

Concentration Value :

Titrated value sufficient to run approximately 10 mini blots.

Conjugation :

(None)

Size :

100 µL

Default Unit :

IF Microscopy Dilution :

1:100

Western Blot Dilution :

1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG1

Gene Name :

Nefm

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-NF-M Monoclonal Antibody was produced by repeated immunizations with recombinant C-terminus part of NF-M expressed in E. coli.

Packing Type :

Dry Ice

Physical State :

Liquid

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Anti-NF-M antibody is directed against NF-M protein. The antibody is protein G purified from concentrated clarified cell culture supernate. Expect reactivity with the following species based on sequence homology: chicken, human, bovine, and mouse. Cross reactivity with NF-M from other species has not been determined.

Species Reactivity :

chicken, human, mouse, rat, bovine

Storage :

Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Subclass :

IgG1

Synonyms :

mouse anti-Neurofilament M Antibody, Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein

Target Species :

Rat

Background :

NF-M antibody detects Neurofilament M protein. Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease. Anti-NF-M antibody is ideal for investigators involved in Cell Signaling and Neuroscience research.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

C-Terminus

Application Text :

IF Microscopy,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P12839

Primary Image Name :

Western blot of Anti-Neurofilament M (Mouse) Antibody - 212-301-D84

more info or order :

Rockland Immunochemicals product webpage