CD3 Antibody | Rockland Immunochemicals 900-C01-B39 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

CD3 Antibody

catalog :

900-C01-B39

quantity :

100 µL

price :

359.00 USD

clonality :

monoclonal

host :

rabbit

conjugate :

nonconjugated

clone name :

SP7

reactivity :

human, mouse, dog

application :

western blot, ELISA, immunohistochemistry, flow cytometry, immunohistochemistry - frozen section

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse; fig 3	Seidel P, Remus M, Delacher M, Grigaravicius P, Reuss D, Frappart L, et al. Epidermal Nbn deletion causes premature hair loss and a phenotype resembling psoriasiform dermatitis. Oncotarget. 2016;7:23006-18 pubmed publisher

product information

Catalog Number :

900-C01-B39

Name :

Anti-CD3 (RABBIT) Monoclonal Antibody - 900-C01-B39

Display Name :

CD3 Antibody

Application Note :

This monoclonal antibody contains TBS/1% BSA and is suitable for ELISA, immunohistochemistry, flow cytometry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 23 kDa in size corresponding to CD3 protein by western blotting in the appropriate cell lysate or extract. Jurkat cell lysates can be used as a control for western and flow. For immunohistochemistry, samples should be formalin fixed and paraffin embedded. Deparaffinize slides using xylene or xylene alternatives and graded alcohols. Staining requires boiling of sections in 10 mM citrate buffer pH 6.0 for 10 min followed by cooling at RT for 20 min. CD3 is a membrane protein. Tonsil tissue can be used as a positive control.

Buffer :

See application note.

Clonality :

Monoclonal

Clone ID :

SP7

Conjugation :

(None)

Size :

100 µL

Default Unit :

ELISA Dilution :

1:10,000

Flow Cytometry Dilution :

1:100

Immunohistochemistry Dilution :

1:150

Western Blot Dilution :

1:200-1:500

Expiration :

Expiration date is one (1) year from date of opening.

Format :

CCS

Gene Name :

CD3E

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-CD3 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues 156-168 of the epsilon chain of human CD3 protein. The hybridoma was produced by fusing New Zealand White rabbit splenocytes and myeloma cells using conventional technology.

Packing Type :

Wet Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.1% (w/v) Sodium Azide

Purity and Specificity :

CD3 Antibody is clarified tissue culture supernatant. The antibody is specific for human CD3 protein. Cross-reactivity is known to occur with baboon, monkey, horse, dog and cat sources of CD3. Cross reactivity with CD3 protein from other sources has not been determined.

Species Reactivity :

human, monkey, horse, dog, cat

Stabilizer :

10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Storage :

Store at 2-8°C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date.

Subclass :

IgG

Synonyms :

rabbit anti-CD3 Antibody, CDE3, CD3-epsilon, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4, epsilon chain, CD3e

Target Species :

Human

Background :

The CDE3 gene encodes the CD3-epsilon polypeptide (also known as CD3, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain and CD3e) , which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. CD3 antibody is ideal for Immunology and Stem Cell research.

General Reference :

Bibollet-Ruche,F., McKinney,B.A., Duverger,A., Wagner,F.H.,Ansari,A.A. and Kutsch,O. (2008) The quality of chimpanzee T-cell activation and simian immunodeficiency virus/human immunodeficiency virus susceptibility achieved via antibody-mediated T-cell receptor/CD3 stimulation is a function of the anti-CD3 antibody isotype. J. Virol. 82 (20), 10271-10278. Takeuchi,K., Yang,H., Ng,E., Park,S.Y., Sun,Z.Y., Reinherz,E.L. and Wagner,G. (2008) Structural and functional evidence that Nck interaction with CD3epsilon regulates T-cell receptor activity. J. Mol. Biol. 380 (4), 704-716. Prado-Garcia,H., Aguilar-Cazares,D., Meneses-Flores,M., Morales-Fuentes,J. and Lopez-Gonzalez,J.S. (2008) Lung carcinomas do not induce T-cell apoptosis via the Fas/Fas ligand pathway but down-regulate CD3 epsilon expression. Cancer Immunol. Immunother. 57 (3), 325-336. Guirado,M., de Aos,I., Orta,T., Rivas,L., Terhorst,C., Zubiaur,M. and Sancho,J. (2002) Phosphorylation of the N-terminal and C-terminal CD3-epsilon-ITAM tyrosines is differentially regulated in T cells. Biochem. Biophys. Res. Commun. 291 (3), 574-581.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

ELISA,Flow Cytometry,Immunohistochemistry,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2019 Price :

359.00 USD

UniProt :

P07766

NCBI :

AAH49847.1

Primary Image Name :

Anti-CD3 Monoclonal Antibody - Immunohistochemistry