Ki-67 Antibody | Rockland Immunochemicals 900-C01-B38 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Ki-67 Antibody

catalog :

900-C01-B38

quantity :

100 µL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SP6

reactivity :

human, rat

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

citations: 1

Reference
Moon H, Donahue L, Choi E, Scumpia P, Lowry W, Grenier J, et al. Melanocyte Stem Cell Activation and Translocation Initiate Cutaneous Melanoma in Response to UV Exposure. Cell Stem Cell. 2017;21:665-678.e6 pubmed publisher

product information

Catalog Number :

900-C01-B38

Name :

Anti-Ki-67 (RABBIT) Monoclonal Antibody - 900-C01-B38

Display Name :

Ki-67 Antibody

Application Note :

This monoclonal antibody contains TBS/1% BSA and is suitable for ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 345 and 395 kDa in size corresponding to Ki-67 protein by western blotting in the appropriate cell lysate or extract. For immunohistochemistry, samples should be formalin fixed and paraffin embedded. Deparaffinize slides using xylene or xylene alternatives and graded alcohols. Staining requires boiling of sections in 10 mM citrate buffer pH 6.0 for 10 min followed by cooling at RT for 20 min. Ki-67 is a nuclear protein. Tonsil tissue can be used as a positive control.

Buffer :

See application note.

Clonality :

Monoclonal

Clone ID :

SP6

Conjugation :

(None)

Size :

100 µL

Default Unit :

ELISA Dilution :

1:10,000

Immunohistochemistry Dilution :

1:200

Western Blot Dilution :

1:200-1:500

Expiration :

Expiration date is one (1) year from date of opening.

Format :

CCS

Gene Name :

MKI67

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to the carboxy terminus of human Ki-67 protein. The hybridoma was produced by fusing New Zealand White rabbit splenocytes and myeloma cells using conventional technology.

Packing Type :

Wet Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.1% (w/v) Sodium Azide

Purity and Specificity :

This product is clarified tissue culture supernatant. The antibody is specific for human Ki-67 protein. Cross-reactivity occurs with rat Ki-67. Cross reactivity with Ki-67 protein from other sources has not been determined.

Species Reactivity :

human, rat

Stabilizer :

10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Storage :

Store at 2-8°C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date.

Subclass :

IgG

Synonyms :

rabbit anti-Ki-67 Antibody, MKI67, Ki-67 nuclear protein

Target Species :

Human

Background :

The MKI67 gene encodes a nuclear protein termed Ki-67 that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. Two isoforms have been reported, with isoform 1 transcribed as the longer transcript. Ki-67 is a nuclear protein predominantly localized in the G1 phase in the perinucleolar region. In later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

C-Terminus

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P46013

NCBI :

CAI16903.1

Primary Image Name :

Anti-Ki-67 Monoclonal Antibody - Immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage