Ubiquitin Hydrolase (UCHL1) Antibody | Rockland Immunochemicals 900-301-E52 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Ubiquitin Hydrolase (UCHL1) Antibody

catalog :

900-301-E52

quantity :

100 µL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

BH7

reactivity :

human, mouse, rat, bovine

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

900-301-E52

Name :

Anti-Ubiquitin Hydrolase (UCHL1) (Mouse) Monoclonal Antibody - 900-301-E52

Display Name :

Ubiquitin Hydrolase (UCHL1) Antibody

Application Note :

Anti-Ubiquitin Hydrolase antibody is suitable for use in ELISA and Western Blot and IF. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 24 kDa in size corresponding to the UCHL1 proteins in Western blot in the appropriate cell lysate or extract.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

BH7

Concentration Value :

Titrated value sufficient to run approximately 10 mini blots.

Conjugation :

(None)

Size :

100 µL

Default Unit :

ELISA Dilution :

1:10,000

IF Microscopy Dilution :

1:500

Western Blot Dilution :

1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG1

Gene Name :

UCHL1

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-UCHL1 Monoclonal Antibody was produced in mouse by repeated immunizations with recombinant full length human UCHL1 extensively purified from E. coli.

Packing Type :

Dry Ice

Physical State :

Liquid

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Anti-UCHL1 antibody is directed against human UCHL1 . The antibody is protein G purified from concentrated clarified cell culture supernate. Reactivity is expected against human, bovine, mouse, and rat sources. Cross reactivity from other sources has not been determined. However, expect reactivity with UCHL1 from most mammalian sources based on sequence homology.

Species Reactivity :

bovine, human, mouse, rat

Stabilizer :

50% (v/v) Glycerol

Storage :

Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Subclass :

IgG1

Synonyms :

mouse anti-UCHL1 Antibody, mouse anti-Ubiquitin Hydrolase Antibody, UCH-L1, Neuron cytoplasmic protein 9.5, PGP 9.5, Ubiquitin thioesterase L1

Target Species :

Human

Background :

UCHL1 antibody recognizes Ubiquitin C-terminal hydrolase 1 (UCHL1) which is also known as ubiquitin carboxyl esterase L1, ubiquitin thiolesterase, neuron-specific protein PGP9.5 and Park5. It was originally identified as a major component of the neuronal cytoplasm from 2-dimensional gel analysis of brain tissues, and was given the name PGP9.5. It was later found that ubiquitin C-terminal hydrolase enzyme activity was associated with the PGP9.5 protein. The ubiquitin C-terminal hydrolases cleave ubiquitin from other molecules. Regulation of the ubiquitin pathway is very important and many disease states are associated with defects in this pathway. Genetic knockout of UCHL1 in mice results in a motor neuron degeneration similar to the spontaneous gracile axonal dystrophy (gad) mutant mice. Point mutations in the UCHL1 gene are associated with some forms of human Parkinson's disease. Since UCHL1 is heavily expressed in neurons, it is released in large amounts following injury or degeneration, so the detection of UCHL1 in CSF and other bodily fluids can be used as a biomarker. Therefore, UCHL1 monoclonal antibody is ideal for investigators involved in Neuroscience.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Application Text :

ELISA,IF Microscopy,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P09936

Primary Image Name :

IF - Ubiquitin Hydrolase (UCHL1)

more info or order :

Rockland Immunochemicals product webpage