Neurofilament H Antibody | Rockland Immunochemicals 900-301-D82 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Neurofilament H Antibody

catalog :

900-301-D82

quantity :

100 µL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

NAP4

reactivity :

human, rat, chicken, bovine

application :

western blot

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

900-301-D82

Name :

Anti-Neurofilament H (Mouse) Antibody - 900-301-D82

Display Name :

Neurofilament H Antibody

Application Note :

Anti-Neurofilament H antibody is suitable for use in Western Blotting and IF. Specific . Expect a band of approximately 200 kDa in size corresponding to the Neurofilament H proteins in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

NAP4

Concentration Value :

Titrated value sufficient to run approximately 10 mini blots.

Conjugation :

(None)

Size :

100 µL

Default Unit :

IF Microscopy Dilution :

1:500

Western Blot Dilution :

1:5000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG1

Gene Name :

NEFH

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-NF-H Antibody was produced by repeated immunizations with purified native bovine NF-H.

Packing Type :

Dry Ice

Physical State :

Liquid

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Anti-NF-H antibody is directed against Neurofilament H. The antibody is protein G purified from concentrated clarified cell culture supernate. Anti-NF-H recognizes phosphorylated NF-H KSP (lysine-serine-proline) type sequences. The antibody has been directly tested for reactivity in chicken and a wide variety of mammals. In some species there is some cross-reactivity with the related phosphorylated KSP sequences found in the related neurofilament subunit NF-M. It recognizes neurofilaments in frozen sections in tissue culture and in formalin fixed sections.

Species Reactivity :

bovine, rat, chicken

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG1

Synonyms :

mouse anti-Neurofilament H Antibody, Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein

Target Species :

Bovine

Background :

NF-H antibody detects Neurofilament H. Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease. Anti-ANeurofilament H antibody is ideal for investigators involved in Cell Signaling and Neuroscience research.

Immunogen Type :

Native Protein

Low Endotoxin :

Sample Size :

Application Text :

IF Microscopy,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P12036

NCBI :

DAA20547.1

Primary Image Name :

Western blot of Anti-Neurofilament H (Mouse) Antibody - 200-301-D82

more info or order :

Rockland Immunochemicals product webpage