Fibrillarin Antibody | Rockland Immunochemicals 900-301-D39 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Fibrillarin Antibody

catalog :

900-301-D39

quantity :

100 µL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

38F3

reactivity :

Saccharomyces cerevisiae S288C, human, mouse, rat

application :

western blot

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

900-301-D39

Name :

Anti-Fibrillarin (Mouse) Antibody - 900-301-D39

Display Name :

Fibrillarin Antibody

Application Note :

Anti-Fibrillarin (Mouse) Antibody is suitable for use in Western Blotting and IF. Specific conditions for reactivity should be optimized by the end user. Expect a band size of approximately 34 kDa in size corresponding to the Fibrillarin/Nop1p proteins.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

38F3

Concentration Value :

Titrated value sufficient to run approximately 10 mini blots.

Conjugation :

(None)

Size :

100 µL

Default Unit :

IF Microscopy Dilution :

1:500

Western Blot Dilution :

1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

NOP1

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-Fibrillarin Monoclonal Antibody was produced in mouse by repeated immunizations with native yeast nuclear protein preparations.

Packing Type :

Dry Ice

Physical State :

Liquid

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Anti-Fibrillarin Antibody is directed against yeast Fibrillarin/Nop1p protein. Fibrillarin antibodies are protein G purified from concentrated clarified cell culture supernate. Reactivity is expected against human, mouse, and rat. Cross-reactivity toward other sources has not be determined. Anti-Fibrillarin antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species.

Species Reactivity :

human, mouse, rat

Stabilizer :

50% (v/v) Glycerol

Storage :

Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Subclass :

IgG1

Synonyms :

mouse anti-Fibrillarin Antibody, rRNA 2'-O-methyltransferase fibrillarin, U3 small nucleolar RNA-associated protein NOP1, Nucleolar protein 1, U3 snoRNA-associated protein NOP1

Target Species :

Yeast

Background :

Fibrillarin Antibody detects Nop1p which was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5kDa), is essential for yeast viability, and is localized in the nucleoli. The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin. Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable. Anti-Fibrillarin Antibody is ideal for investigators involved in Cell Proliferation and Cancer Research.

Immunogen Type :

Native Protein

Low Endotoxin :

Sample Size :

Application Text :

IF Microscopy,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P15646

NCBI :

AAA34816.1

Primary Image Name :

Immunofluorescence of Anti-Fibrillarin (Mouse) Antibody

more info or order :

Rockland Immunochemicals product webpage