domestic rabbit polyclonal
reactivity: Saccharomyces cerevisiae S288C
application: western blot, ELISA
reactivity: Saccharomyces cerevisiae S288C
application: western blot, ELISA
Both the antiserum and IgG fractions of anti-Carboxypeptidase Y (Baker's Yeast) are shown to detect under reducing conditions of SDS-PAGE the 61,000 dalton enzyme in cellular extracts. Approximately 10 µg of total protein is loaded per lane. A 1:5,000 dilution of the primary antibody is used followed by detection using HRP Goat-a-Rabbit IgG [H&L] (611-1302) diluted 1:4,000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results.
quantity: 2 mL
price:
to the supplier
domestic rabbit polyclonal
reactivity: Saccharomyces cerevisiae S288C
application: western blot, ELISA
reactivity: Saccharomyces cerevisiae S288C
application: western blot, ELISA
Western Blot of Rabbit anti-Carboxypeptidase Y (Baker's Yeast) antibody. Lane 1: cellular extracts. Lane 2: none. Load: 10 µg per lane. Primary antibody: Carboxypeptidase Y antibody at 1:5000 for overnight at 4°C. Secondary antibody: HRP Goat-a-Rabbit IgG [H&L] secondary antibody at 1:4,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 61 kDa, 61 kDa for Carboxypeptidase Y. Other band(s): none.
quantity: 100 µg
price:
to the supplier
domestic rabbit polyclonal
reactivity: Saccharomyces cerevisiae S288C
conjugate: biotin
application: western blot, ELISA
reactivity: Saccharomyces cerevisiae S288C
conjugate: biotin
application: western blot, ELISA
domestic rabbit polyclonal
reactivity: Saccharomyces cerevisiae S288C
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
reactivity: Saccharomyces cerevisiae S288C
conjugate: HRP
application: western blot, ELISA, immunohistochemistry