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company name :
Rockland Immunochemicals
product type :
antibody
product name :
Fab Biotin Antibody Alkaline Phosphatase Conjugated
catalog :
800-105-098
quantity :
1 mg
clonality :
polyclonal
host :
domestic goat
conjugate :
AP
application :
western blot, ELISA, immunohistochemistry
more info or order :
Rockland Immunochemicals product webpage
product information
Catalog Number :
800-105-098
Name :
Fab Anti-BIOTIN (GOAT) Antibody Alkaline Phosphatase Conjugated - 800-105-098
Display Name :
Fab Biotin Antibody Alkaline Phosphatase Conjugated
Application Note :
Fab Anti-Biotin Antibody Alkaline Phosphatase Conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Specific conditions for reactivity should be optimized by the end user.
Buffer :
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Clonality :
Polyclonal
Concentration Value :
1.0 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
ALP
Size :
1 mg
Default Unit :
mg
ELISA Dilution :
1:2,000 - 1:10,000
Immunohistochemistry Dilution :
1:200 - 1:1,000
Western Blot Dilution :
1:500 - 1:2,500
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG Fab
Host Animal :
Goat
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
Packing Type :
Wet Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
Fab Biotin antibody was prepared from monospecific antiserum by immunoaffinity chromatography using biotin coupled to sepharose beads followed by papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum.  No reaction was observed against anti-papain or anti-Goat IgG F(c).
Stabilizer :
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Storage :
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Synonyms :
Goat Fab Anti-Biotin Antibody Alkaline Phosphatase Conjugation, Goat Fab Anti-Biotin HRP Conjugated Antibody
Background :
Fab Biotin antibody recognizes Biotin. Biotin, also known as vitamin H or coenzyme R, is a water-soluble B-vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis.
ABS Reagent Type :
Biotin
Immunogen Type :
Other
Low Endotoxin :
No
Sample Size :
No
Application Text :
ELISA,Immunohistochemistry,Western Blot,
Category :
GFP and Epitopes
Conjugation Name :
Alkaline Phosphatase (Calf Intestine)
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.