Fab Biotin Antibody Peroxidase Conjugated | Rockland Immunochemicals 800-103-098 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Fab Biotin Antibody Peroxidase Conjugated

catalog :

800-103-098

quantity :

1 mg

clonality :

polyclonal

host :

domestic goat

conjugate :

HRP

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

800-103-098

Name :

Fab Anti-BIOTIN (GOAT) Antibody Peroxidase Conjugated - 800-103-098

Display Name :

Fab Biotin Antibody Peroxidase Conjugated

Application Note :

Fab Anti-Biotin Antibody Peroxidase Conjugated is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

HRP

Size :

1 mg

Default Unit :

ELISA Dilution :

1:340,000

Immunohistochemistry Dilution :

1:1,000 - 1:5,000

Western Blot Dilution :

1:2,000 - 1:20,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG Fab

Host Animal :

Goat

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Packing Type :

Ambient

Physical State :

Lyophilized

Preservative :

0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Purity and Specificity :

Fab Biotin antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).

Reconstitution Buffer :

Restore with deionized water (or equivalent)

Reconstitution Volume :

1.0 mL

Stabilizer :

10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Storage :

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

Goat Fab Anti-Biotin Antibody Peroxidase Conjugation, Goat Fab Anti-Biotin HRP Conjugated Antibody

Background :

Fab Biotin antibody recognizes Biotin. Biotin, also known as vitamin H or coenzyme R, is a water-soluble B-vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis.

ABS Reagent Type :

Biotin

Immunogen Type :

Other

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Category :

GFP and Epitopes

Conjugation Name :

Peroxidase (Horseradish)

more info or order :

Rockland Immunochemicals product webpage