MYC Epitope Tag Antibody
Rockland Immunochemicals
catalog: 600-401-381

domestic rabbit polyclonal
application: western blot, ELISA, immunohistochemistry
citations: 2

Anti-Myc epitope tag polyclonal antibody detects both AMINO and CARBOXY terminal linked Myc-tagged recombinant proteins by western blot. Polyclonal rabbit host anti-Myc epitope tag antibody was diluted to 1.0 µg/ml to detect either recombinant protein.  4-20% gradient gels were used to resolve the proteins by SDS-PAGE.  The proteins were transferred to nitrocellulose using standard methods.  After blocking, the membranes were probed with the primary antibody overnight at 4°C followed by washes and reaction with a 1:10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 (code 611-132-122) for 45 min at room temperature (Green, 800 nm channel).   Pre-stained molecular weight markers are also shown (lane M, Red, 700 nm channel).  LICOR's Odyssey® Infrared Imaging System was used to scan and process the image.  Other detection systems will yield similar results.
quantity: 100 µg
price:
to the supplier

c-Myc Antibody
Rockland Immunochemicals
catalog: 100-401-224

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA

Anti-c-myc is shown to detect c-myc in infected cells (lanes 2-4) showing a 66 kDa band. No band specific staining is observed in uninfected cells (lane 1). Detection occurs using a 1:250 dilution of antibody followed by 1:2,000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1’. Other detection systems will yield similar results.
quantity: 100 µL
price:
to the supplier

MYC Epitope Tag Antibody Peroxidase Conjugated
Rockland Immunochemicals
catalog: 600-403-381

domestic rabbit polyclonal
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

quantity: 100 µg
price:
to the supplier

MYC Epitope Tag Antibody Biotin Conjugated
Rockland Immunochemicals
catalog: 600-406-381

domestic rabbit polyclonal
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

Anti-Myc epitope tag polyclonal antibody detects Myc-tagged recombinant proteins by western blot.  Polyclonal rabbit-anti-Myc epitope tag at 0.5-1.0 µg/ml was used to detect 1.0 ug of recombinant protein containing the Myc epitope tag.  A 4-20% gradient gel was used to separate the protein by SDS-PAGE.  The protein was transferred to nitrocellulose using standard methods.  After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] (code 611-132-122) for 30 min at room temperature.   LICOR's Odyssey® Infrared Imaging System was used to scan and process the image.  Other detection systems will yield similar results.
quantity: 100 µg
price:
to the supplier

MYC Epitope Tag Antibody Dylight™ 488 Conjugated
Rockland Immunochemicals
catalog: 600-441-381

domestic rabbit polyclonal
conjugate: DyLight488
application: western blot

Properties of Dylight™ Conjugates.
quantity: 100 µg
price:
to the supplier

MYC Epitope Tag Antibody Dylight™ 549 Conjugated
Rockland Immunochemicals
catalog: 600-442-381

domestic rabbit polyclonal
conjugate: DyLight549
application: western blot

DyLight™ dyes can be used for two-color Western Blot detection with low background and high signal.  Anti-tubulin was detected using a DyLight™ 549 conjugate.  Anti-TNFa was detected using a DyLight™ 649 conjugate. The image was captured using the Typhoon™ 9410 Imaging System.
quantity: 100 µg
price:
to the supplier

MYC Epitope Tag Antibody Dylight™ 649 Conjugated
Rockland Immunochemicals
catalog: 600-443-381

domestic rabbit polyclonal
conjugate: DyLight649
application: western blot

quantity: 100 µg
price:
to the supplier

MYC Epitope Tag Antibody Dylight™ 680 Conjugated
Rockland Immunochemicals
catalog: 600-444-381

domestic rabbit polyclonal
conjugate: DyLight680
application: western blot

quantity: 100 µg
price:
to the supplier

MYC Epitope Tag Antibody Dylight™ 800 Conjugated
Rockland Immunochemicals
catalog: 600-445-381

domestic rabbit polyclonal
conjugate: DyLight800
application: western blot

quantity: 100 µg
price:
to the supplier

MYC Epitope Tag Antibody Dylight™ 405 Conjugated
Rockland Immunochemicals
catalog: 600-446-381

domestic rabbit polyclonal
conjugate: DyLight 405
application: western blot

quantity: 100 µg
price:
to the supplier