VEGF Antibody Peroxidase Conjugated
Rockland Immunochemicals
catalog: 209-403-B99

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, immunohistochemistry

Western Blot showing detection of Recombinant Human VEGF-165. 50ng of protein (Lane 1) was run on a 4-20% gel and transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) 30 min at 20°C, Anti-VEGF-165 (RABBIT) Antibody Peroxidase Conjugate (p/n 209-403-B99) secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) and imaged using the Bio-Rad VersaDoc® 4000 MP. Arrow indicates correct 19 kDa molecular weight position expected for rH-VEGF-165.
quantity: 100 µg
price:
to the supplier

VEGF-165 Antibody
Rockland Immunochemicals
catalog: 209-401-B99

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

Rockland's anti-Human VEGF-165 antibody shows detection of a band ~22 kDa in size corresponding to recombinant human VEGF-165. This isoform of VEGF tends to form a disulfide linked homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reaction with FemtoMax™ chemiluminescent substrate. Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).
quantity: 100 µg
price:
to the supplier

VEGF-121 Antibody
Rockland Immunochemicals
catalog: 209-401-C23

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

Anti-human VEGF-121 by western blot shows detection of recombinant Human VEGF-121 raised in E.coli. Recombinant (0.1 µg, 28.4 kDa) protein was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Rockland's, Inc. Anti-Human VEGF-121. After washing, membrane was probed with secondary antibody Dylight™ 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody (611-143-122) diluted 1:20,000 in blocking buffer (p/n MB-070) for 30 min. at RT. Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system.
quantity: 100 µg
price:
to the supplier

VEGF Antibody Biotin Conjugated
Rockland Immunochemicals
catalog: 209-406-B99

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

quantity: 100 µg
price:
to the supplier

Recombinant Anti-VEGF Fab Antibody
Rockland Immunochemicals
catalog: 400-001-MT4

recombinant (Lucentis)
reactivity: human
application: western blot, ELISA, flow cytometry

Western Blot using Anti-human VEGF Fab fragment Antibody to detect human VEGF165 protein. MW: Molecular Weight Ladder. Lanes 1, 3, and 5: VEGF165 reduced. Lanes 2, 4, and 6: VEGF165 non-reduced. Protein Load: Lane 1-2: 200ng. Lane 3-4: 40ng. Lane 5-6: 8ng. Primary Antibody: Anti-VEGF Fab fragment at 1.0µg/mL for 2 hours at 4°C. Secondary Antibody: F(ab')2 Human IgG F(ab')2 Antibody Peroxidase Conjugated Pre-Adsorbed (p/n 709-1318) at 1:5,000 dilution was used. The VEGF165 protein has a calculated MW of 19.2kDa. As a result of glycosylation, the protein migrates as 24kDa (monomer) under reducing (R) condition, and 43-50kDa (homodimer) under non-reducing (NR) condition (SDS-PAGE).
quantity: 50
price: 465.00 USD
to the supplier

Recombinant Anti-VEGF RTH258 scFv Antibody
Rockland Immunochemicals
catalog: 400-001-MT5

recombinant (Abz5)
reactivity: human
application: western blot, ELISA, flow cytometry

Western Blot using Humanized Recombinant Brolucizumab/RTH258 Anti-human VEGF ScFv to detect structured and denatured human VEGF165 protein. MW: Molecular Weight Ladder. Lanes 1,3, and 5: VEGF165 reduced. Lanes 2, 4, and 6: VEGF165 non-reduced. Protein Load: Lane 1-2: 200ng. Lane 3-4: 40ng. Lane 5-6: 8ng. Primary Antibody: FLAG-tagged Humanized Recombinant Brolucizumab/RTH258 Anti-human VEGF ScFv at 1.0µg/mL for 2 hours at 4°C. Secondary Antibody: Antibody for the detection of FLAG™ conjugated proteins (MOUSE) Monoclonal Antibody Peroxidase Conjugated (p/n 200-303-383) at 1:5000 for 2 hours at 4°C. The VEGF165 protein has a calculated MW of 19.2kDa. As a result of glycosylation, the protein migrates as 24kDa (monomer) under reducing (R) condition, and 43-50kDa (homodimer) under non-reducing (NR) condition (SDS-PAGE).
quantity: 50
price: 465.00 USD
to the supplier