mouse monoclonal (BP53-12)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, chromatin immunoprecipitation
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, chromatin immunoprecipitation
Western blotting using anti-p53. HeLa whole cell lysate (lane 1), cytosol fraction (lane 2) and nuclear extract (lane 3) (15 ?g) were separated by 10% SDS-PAGE and transferred to nitrocellulose membrane. The membrane was blocked with 3% milk/TBST for 1 h at room temperature followed by incubation with Rockland's Protein A purified Mab anti-p53 overnight at 4° C diluted 1:1,500 in blocking solution. The membrane was washed 3X with TBST and then incubated with a 1:2,000 dilution of HRP Goat-a-Mouse IgG diluted in blocking buffer for 1 h at room temperature. After final washes the proteins reactive on the membrane were detected using ECL. Other detection systems will yield similar results. Personnel Communication. Kuldeep Patel, Loyola University.
quantity: 100 µg
price:
to the supplier
domestic rabbit polyclonal
reactivity: human, rat
application: western blot
reactivity: human, rat
application: western blot
Western Blot of Rabbit anti-p53 pS392 antibody. Lane 1: rat brain nuclear fraction lysate (control). Lane 2: ?-Ptase. Load: 10 µg per lane. Primary antibody: p53 pS392 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 53 kDa for p53 pS392. Other band(s): none.
quantity: 100 µL
price:
to the supplier
domestic rabbit polyclonal
reactivity: human
application: western blot, chromatin immunoprecipitation
reactivity: human
application: western blot, chromatin immunoprecipitation
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
reactivity: human
application: western blot, ELISA
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
reactivity: human
application: western blot, ELISA