domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry
citations: 1
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry
citations: 1
Immunohistochemistry using Rockland's polyclonal TNFa antibody showing staining of formalin/PFA-fixed paraffin-embedded sections of human artery tissue sections. Sections were fixed in formaldehyde and subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0). Slides were blocked for ten minutes with 1.5% serum. Primary antibody was diluted 1:100 and incubated with samples for 24 hours at 4°C. HRP-conjugated goat anti-rabbit antibody was used as the secondary antibody.
quantity: 1
price: 1,292.00 USD
to the supplier
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
reactivity: human
application: western blot, ELISA, immunohistochemistry
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
reactivity: human
application: western blot, ELISA, immunohistochemistry
recombinant (Abz520)
reactivity: human
application: western blot, ELISA
reactivity: human
application: western blot, ELISA
Western Blot using Adalimumab, Anti-human TNF alpha Fab fragment to detect human TNA alpha. Lanes 1, 3, and 5: TNF alpha reduced. Lanes 2, 4, 6: TNF alpha non-reduced. MW: Molecular Weight Ladder. Protein Load: Lane 1-2: 200ng. Lane 3-4: 40ng. Lane 5-6: 8ng. Primary Antibody: Anti-TNF alpha Fab fragment Antibody at 1.0µg/mL for 2 hours at 4°C. Secondary Antibody: F(ab')2 Human IgG F(ab')2 Antibody Peroxidase Conjugated Pre-Adsorbed (p/n 709-1318) at 1:5,000 dilution was used. The protein has a calculated MW of 18.3kDa. The protein migrates as 18.2kDa under reducing (R) and non-reducing condition (SDS-PAGE).
quantity: 50
price: 465.00 USD
to the supplier