F(ab')2 Rat IgG (H&L) Antibody Phycoerythrin conjugated | Rockland Immunochemicals 712-408-002 product information

product summary

company name :

Rockland Immunochemicals

product type :

secondary antibody

product name :

F(ab')2 Rat IgG (H&L) Antibody Phycoerythrin conjugated

catalog :

712-408-002

quantity :

500 µg

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

712-408-002

Name :

F(ab')2 Anti-RAT IgG [H&L] (RABBIT) Antibody Phycoerythrin conjugated - 712-408-002

Display Name :

F(ab')2 Rat IgG (H&L) Antibody Phycoerythrin conjugated

Application Note :

F(ab')2 Anti-Rat Phycoerythrin Conjugated Secondary Antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

0.5 mg/mL

Concentration Definition :

by absorbance = 82.0 at 565 nm

Conjugation :

RPE

Size :

500 µg

Default Unit :

µg

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG F(ab')2

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Rat IgG whole molecule

Packing Type :

Ambient

Physical State :

Lyophilized

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by pepsin digestion and chromatographic separation. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Rabbit Serum, Rat IgG and Rat Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).

Reconstitution Buffer :

Restore with deionized water (or equivalent)

Reconstitution Volume :

1.0 mL

Stabilizer :

10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Storage :

Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.

Synonyms :

Rabbit F(ab')2 Anti-Rat IgG Phycoerythrin Conjugated Antibody, Rabbit Fab2 Anti-Rat IgG Antibody Phycoerythrin Conjugation

Target Species :

Rat

Background :

F(ab')2 Anti-Rat IgG (H&L) Antibody generated in rabbit detects immunoglobulin g from Rat, both heavy and light chains of the antibody molecule are present. Each IgG has two antigen binding sites. Representing approximately 75% of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays. This F(ab')2 Anti-Rat IgG Antibody is conjugated to Phycoerythrin.

Low Endotoxin :

Sample Size :

Secondary Antibody Specificity :

IgG (H&L)

Category :

Secondary Antibodies

Conjugation Name :

R-Phycoerythrin (RPE)

more info or order :

Rockland Immunochemicals product webpage