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company name :
Rockland Immunochemicals
product type :
secondary antibody
product name :
F(ab')2 Rat IgG (H&L) Antibody Alkaline Phosphatase Conjugated
catalog :
712-405-002
quantity :
500 µg
more info or order :
Rockland Immunochemicals product webpage
product information
Catalog Number :
712-405-002
Name :
F(ab')2 Anti-RAT IgG [H&L] (RABBIT) Antibody Alkaline Phosphatase Conjugated - 712-405-002
Display Name :
F(ab')2 Rat IgG (H&L) Antibody Alkaline Phosphatase Conjugated
Application Note :
F(ab')2 Anti-Rat IgG Antibody has been assayed against 1.0 ug of Rat IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for F(ab')2 Anti-Rat IgG Antibody.
Buffer :
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Clonality :
Polyclonal
Concentration Value :
1.0 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
ALP
Size :
500 µg
Default Unit :
µg
ELISA Dilution :
1:2,000 - 1:10,000
Immunohistochemistry Dilution :
1:200 - 1:1,000
Western Blot Dilution :
1:500 - 1:2,500
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG F(ab')2
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Rat IgG whole molecule
Packing Type :
Wet Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.1% (w/v) Sodium Azide
Purity and Specificity :
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by pepsin digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Rabbit Serum, Rat IgG and Rat Serum.  No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).
Stabilizer :
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Storage :
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Synonyms :
Rabbit F(ab')2 Anti-Rat IgG Alkaline Phosphatase Conjugated Antibody, Rabbit F(ab')2 Anti-Rat IgG Antibody alk phos Conjugation
Target Species :
Rat
Background :
F(ab')2 Anti-Rat IgG Antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. F(ab')2 Anti-Rat IgG Antibody is ideal for investigators in Cancer, Immunology, and Microbiology research.
Low Endotoxin :
No
Sample Size :
No
Secondary Antibody Specificity :
IgG (H&L)
Application Text :
ELISA,Immunohistochemistry,Western Blot,
Other :
User Optimized
Category :
Secondary Antibodies
Conjugation Name :
Alkaline Phosphatase (Calf Intestine)
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
Info@rockland-inc.com
https://rockland-inc.com
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.