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company name :
Rockland Immunochemicals
product type :
secondary antibody
product name :
F(ab')2 Mouse IgG (H&L) Antibody Alkaline Phosphatase Conjugated
catalog :
710-1502
quantity :
500 µg
more info or order :
Rockland Immunochemicals product webpage
product information
Catalog Number :
710-1502
Name :
F(ab')2 Anti-MOUSE IgG [H&L] (GOAT) Antibody Alkaline Phosphatase Conjugated - 710-1502
Display Name :
F(ab')2 Mouse IgG (H&L) Antibody Alkaline Phosphatase Conjugated
Application Note :
This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature.  A working dilution of 1:600 to 1:2,750 of the reconstitution concentration is suggested for this product. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Buffer :
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Clonality :
Polyclonal
Concentration Value :
0.6 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
ALP
Size :
500 µg
Default Unit :
µg
ELISA Dilution :
1:2,000 - 1:10,000
Immunohistochemistry Dilution :
1:200 - 1:1,000
Western Blot Dilution :
1:500 - 1:2,500
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG F(ab')2
Host Animal :
Goat
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Mouse IgG whole molecule
Packing Type :
Wet Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Mouse IgG and Mouse Serum.  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c).
Stabilizer :
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Storage :
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Synonyms :
Goat F(ab')2 Anti-Mouse IgG Antibody alkaline phosphatase Conjugation, Goat Fab2 Anti-Mouse IgG alk phos Conjugated Antibody
Target Species :
Mouse
Background :
F(ab')2 Anti-Mouse IgG (H&L) Alkaline Phosphatase Antibody generated in goat was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
Low Endotoxin :
No
Sample Size :
No
Secondary Antibody Specificity :
IgG (H&L)
Application Text :
ELISA,Immunohistochemistry,Western Blot,
Other :
User Optimized
Category :
Secondary Antibodies
Conjugation Name :
Alkaline Phosphatase (Calf Intestine)
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
Info@rockland-inc.com
https://rockland-inc.com
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.