domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
reactivity: human
application: western blot, ELISA, immunohistochemistry
Immunohistochemistry of Rabbit Anti-TLR4 Antibody. Tissue: K562 cells. Fixation: paraformaldehyde fixed. Antigen retrieval: not required. Primary antibody: TLR 4 antibody at 1:500 for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: TLR-4 is cytoplasmic. Staining: TLR 4 as precipitated brown signal with hematoxylin purple nuclear counterstain.
quantity: 100 µg
price:
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry
reactivity: human, mouse
application: western blot, immunohistochemistry
Western Blot of rabbit anti-TLR4 antibody. Lane 1: Partial Recombinant Mouse TLR4. Primary antibody: TLR4 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Goat anti-rabbit IgG HRP secondary antibody at 1:10,000 for 45 min at RT. Block: 5% Blotto overnight 4°C. Predicted/Observed size:95.6kDa/75-80kD. Other band(s): none.
quantity: 100 µg
price:
to the supplier
mouse monoclonal (76B357.1)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, flow cytometry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, flow cytometry
Western Blot of Mouse Anti-TLR4 CD284 antibody. Lane A: human intestine. Lane B: mouse intestine. Lane C: rat intestine lysate. Primary antibody: TLR4 antibody at 2 µg/mL for tissue Lane A and at 6 µg/mL for Lanes B and C overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 80 kDa for TLR4 CD284. Other band(s): none.
Western Blot of Mouse Anti-TLR4 CD284 antibody. Lane A: human intestine. Lane B: mouse intestine. Lane C: rat intestine lysate. Primary antibody: TLR4 antibody at 2 µg/mL for tissue Lane A and at 6 µg/mL for Lanes B and C overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 80 kDa for TLR4 CD284. Other band(s): none.
Flow Cytometry of Mouse Anti-TLR4 CD284 antibody. Cells: Mouse splenocytes. Stimulation: none. Primary Antibody: Anti-TLR3 antibody at 0.5 ug/10^6 cells of antibody (red) and the isotype control (green). Secondary Antibody: Goat anti-mouse IgG1 PE conjugate (BD).
quantity: 100 µg
price:
to the supplier
domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry
reactivity: human
application: ELISA, immunohistochemistry
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
reactivity: human
application: western blot, ELISA, immunohistochemistry
Immunohistochemistry of Rabbit Anti-TLR4 Antibody. Tissue: Human Tonsil. Antigen retrieval: HIER using Citrate Buffer for 20min. Primary Antibody: Anti-TLR4 at 1:100 at RT for 30mins. Secondary Antibody: Ready-to-Use Anti-Rabbit Poly-HRP-IgG at RT for 8mins. Stain: DAB. Counterstain: Hematoxylin. Light cytoplasmic staining in random cells near blood vessels is consistent with what is expected of TLR staining in the tonsil.
quantity: 100 µg
price:
to the supplier