F(ab')2 Human IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed | Rockland Immunochemicals 709-1516 product information

product summary

company name :

Rockland Immunochemicals

product type :

secondary antibody

product name :

F(ab')2 Human IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

catalog :

709-1516

quantity :

500 µg

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

709-1516

Name :

F(ab')2 Anti-HUMAN IgG (H&L) (GOAT) Antibody Alkaline Phosphatase Conjugated Min X Bv Hs Ms & Rt Serum Proteins - 709-1516

Display Name :

F(ab')2 Human IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

Application Note :

F(ab')2 Anti-Human IgG (H&L) Alkaline Phosphatase Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Buffer :

0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0

Clonality :

Polyclonal

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

ALP

Size :

500 µg

Default Unit :

µg

ELISA Dilution :

1:2,000 - 1:10,000

Immunohistochemistry Dilution :

1:200 - 1:1,000

Western Blot Dilution :

1:500 - 1:2,500

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG F(ab')2

Host Animal :

Goat

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Human IgG whole molecule

Packing Type :

Wet Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.1% (w/v) Sodium Azide

Purity and Specificity :

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Human IgG and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Mouse and Rat Serum Proteins.

Stabilizer :

10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Storage :

Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Synonyms :

Goat F(ab')2 Anti-Human IgG alkaline phosphatase Conjugated Antibody Pre-Adsorbed, Goat F(ab')2 Anti-Human IgG Antibody alk phos Conjugation

Target Species :

Human

Background :

F(ab')2 Anti-Human IgG (H&L) Alkaline Phosphatase Antibody generated in goat detects immunoglobulin g from human, both heavy and light chains of the antibody molecule are present. Each IgG has two antigen binding sites. Representing approximately 75% of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

Low Endotoxin :

Sample Size :

Secondary Antibody Specificity :

IgG (H&L)

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Other :

User Optimized

Category :

Secondary Antibodies

Conjugation Name :

Alkaline Phosphatase (Calf Intestine)

more info or order :

Rockland Immunochemicals product webpage