secondary antibody

F(ab')2 Human IgG F(ab')2 Antibody Pre-Adsorbed

709-1118

1 mg

F(ab')2 Anti-HUMAN IgG F(ab')2 (GOAT) Antibody Min X Bv Hs Ms & Rt Serum Proteins - 709-1118

F(ab')2 Human IgG F(ab')2 Antibody Pre-Adsorbed

Antibody Anti-Human IgG F(ab')2 is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Polyclonal

1.0 mg/mL

by UV absorbance at 280 nm

(None)

1 mg

mg

1:25,000

1:500 - 1:2,500

1:1,000 - 1:5,000

Expiration date is one (1) year from date of opening.

IgG F(ab')2

Goat

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Anti-Human IgG was produced by repeated immunization with human IgG F(ab')2 fragment in goat.

Wet Ice

Liquid (sterile filtered)

0.01% (w/v) Sodium Azide

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG, Human IgG F(ab’)2 and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG F(c) or Bovine, Horse, Mouse and Rat Serum Proteins.

Human

Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Goat F(ab')2 Anti-Human IgG F(ab')2 Antibody Pre-Adsorbed, Goat F(ab')2 Anti-Human IgG F(ab')2 Antibody

Human

F(ab')2 Anti-Human IgG F(ab')2 Antibody generated in goat detects F(ab')2 from human. Representing approximately 75% of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

Native Protein

No

No

IgG F(ab')2

ELISA,Immunohistochemistry,Western Blot,

User Optimized

Secondary Antibodies

Unconjugated

SDS-PAGE of Goat F(ab')2 Anti-Human IgG F(ab')2 Antibody