F(ab')2 Horse IgG (H&L) Antibody Phycoerythrin conjugated | Rockland Immunochemicals 708-408-002 product information

product summary

company name :

Rockland Immunochemicals

product type :

secondary antibody

product name :

F(ab')2 Horse IgG (H&L) Antibody Phycoerythrin conjugated

catalog :

708-408-002

quantity :

500 µg

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

708-408-002

Name :

F(ab')2 Anti-HORSE IgG [H&L] (RABBIT) Antibody Phycoerythrin conjugated - 708-408-002

Display Name :

F(ab')2 Horse IgG (H&L) Antibody Phycoerythrin conjugated

Application Note :

This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

0.5 mg/mL

Concentration Definition :

by absorbance = 82.0 at 565 nm

Conjugation :

RPE

Size :

500 µg

Default Unit :

µg

Flow Cytometry Dilution :

1:100-1:250

IF Microscopy Dilution :

1:100-1:250

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG F(ab')2

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Horse IgG whole molecule

Packing Type :

Ambient

Physical State :

Lyophilized

Purity and Specificity :

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-phycoerythrin, anti-Rabbit Serum, Horse IgG and Horse Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).

Reconstitution Buffer :

Restore with deionized water (or equivalent)

Reconstitution Volume :

1.0 mL

Species Reactivity :

horse

Storage :

Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.

Synonyms :

Rabbit F(ab')2 Anti-Horse IgG Antibody Phycoerythrin Conjugation, Rabbit F(ab')2 Anti-Horse IgG Phycoerythrin Conjugated Antibody, Rabbit Fab2 Anti-Horse IgG PE Conjugated Antibody

Target Species :

Horse

Background :

F(ab')2 Anti-Horse IgG Phycoerythrin Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

Low Endotoxin :

Sample Size :

Secondary Antibody Specificity :

IgG (H&L)

Application Text :

Flow Cytometry,IF Microscopy,

Other :

Flow Cytometry: 1:100-1:250

Category :

Secondary Antibodies

Conjugation Name :

R-Phycoerythrin (RPE)

Primary Image Name :

Fab2 Horse IgG Antibody Phycoerythrin Conjugated - Dot Blot

more info or order :

Rockland Immunochemicals product webpage