F(ab')2 Goat IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed | Rockland Immunochemicals 705-703-125 product information

product summary

company name :

Rockland Immunochemicals

product type :

secondary antibody

product name :

F(ab')2 Goat IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

catalog :

705-703-125

quantity :

500 µg

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

705-703-125

Name :

F(ab')2 Anti-GOAT IgG [H&L] (DONKEY) Antibody Peroxidase Conjugated Min X Ch GP Ham Hs Hu Ms Rb & Rt Serum Proteins - 705-703-125

Display Name :

F(ab')2 Goat IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Application Note :

This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

HRP

Size :

500 µg

Default Unit :

µg

ELISA Dilution :

1:10,000 - 1:50,000

Immunohistochemistry Dilution :

1:500 - 1:5,000

Western Blot Dilution :

1:2,000 - 1:10,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG F(ab')2

Host Animal :

Donkey

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Goat IgG whole molecule

Packing Type :

Ambient

Physical State :

Lyophilized

Preservative :

0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Purity and Specificity :

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Goat IgG and Goat Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Rat Serum Proteins.

Reconstitution Buffer :

Restore with deionized water (or equivalent)

Reconstitution Volume :

500 µL

Stabilizer :

10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Storage :

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

Donkey F(ab')2 Anti-Goat IgG Antibody Peroxidase Conjugation, Donkey Fab2 Anti-Goat IgG HRP Conjugated Antibody

Target Species :

Goat

Background :

F(ab')2 Anti-Goat IgG Peroxidase Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

Low Endotoxin :

Sample Size :

Secondary Antibody Specificity :

IgG (H&L)

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Other :

User Optimized

Category :

Secondary Antibodies

Conjugation Name :

Peroxidase (Horseradish)

Primary Image Name :

ELISA Results of F(ab')2 Donkey Anti-Goat IgG MX8 Peroxidase Conjugated

more info or order :

Rockland Immunochemicals product webpage