F(Ab')2 Biotin Antibody Alkaline Phosphatase Conjugated | Rockland Immunochemicals 700-105-098 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

F(Ab')2 Biotin Antibody Alkaline Phosphatase Conjugated

catalog :

700-105-098

quantity :

1 mg

clonality :

polyclonal

host :

domestic goat

conjugate :

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

700-105-098

Name :

F(ab')2 Anti-BIOTIN (GOAT) Antibody Alkaline Phosphatase Conjugated - 700-105-098

Display Name :

F(Ab')2 Biotin Antibody Alkaline Phosphatase Conjugated

Application Note :

This product has been assayed against 1.0 ug of Biotin conjugated IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,000 of the reconstitution concentration is suggested for this product.

Buffer :

0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0

Clonality :

Polyclonal

Concentration Value :

0.5 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

ALP

Size :

1 mg

Default Unit :

ELISA Dilution :

1:500-1:2,000

Immunohistochemistry Dilution :

1:500-1:1,000

Western Blot Dilution :

1:1,000-1:5,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG F(ab')2

Host Animal :

Goat

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Packing Type :

Wet Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum and Biotinylated IgG. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).

Stabilizer :

10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Storage :

Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Synonyms :

Goat F(ab')2 Anti-Biotin alkaline phosphatase Conjugated Antibody, Goat F(ab')2 Anti-Biotin Antibody alkaline phosphatase Conjugation, Goat Fab2 Anti-Biotin Antibody alk phos Conjugation

Background :

F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays. Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.

ABS Reagent Type :

Biotin

Immunogen Type :

Other

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Category :

GFP and Epitopes

Conjugation Name :

Alkaline Phosphatase (Calf Intestine)

more info or order :

Rockland Immunochemicals product webpage