mouse monoclonal (3N2D9)
reactivity: human
application: western blot
reactivity: human
application: western blot
Western Blot of Human MIP 3? (Mouse) Antibody Lane 1: human recombinant MIP-3a Lane 2: mouse recombinant MIP-3a Primary antibody: Human MIP 3? (RAT) Antibody at 0.5 ug/ml for overnight at 4°C Secondary antibody: IRDye800™ goat anti-rabbit at 1:10,000 for 45 min at RT Block: 5% BLOTTO overnight at 4°C
quantity: 100 µg
price:
to the supplier
mouse monoclonal (3N2D9)
reactivity: human
conjugate: biotin
application: western blot
reactivity: human
conjugate: biotin
application: western blot
Western Blot of Human MIP 3? (Mouse) Antibody Lane 1: human recombinant MIP-3a Lane 2: mouse recombinant MIP-3a Primary antibody: Human MIP 3? (RAT) Antibody at 0.5 ug/ml for overnight at 4°C Secondary antibody: IRDye800™ goat anti-rabbit at 1:10,000 for 45 min at RT Block: 5% BLOTTO overnight at 4°C
quantity: 100 µg
price:
to the supplier
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
reactivity: human
application: western blot, ELISA
Western blot using Rockland's anti-Human MIP-3a antibody shows detection of a band ~26 kDa in size corresponding to recom-binant human MIP-3a (lane 1). The identity of the lower molecular weight band is unknown. Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reaction with FemtoMax™ chemiluminescent substrate. Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).
quantity: 100 µg
price:
to the supplier
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
Western Blot of Peroxidase Conjugated Rabbit anti-MIP-3? antibody. Lane 1: Human MIP-3?. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase rabbit secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 11 kDa, 11 kDa for Human MIP-3?. Other band(s): None.
quantity: 100 µg
price:
to the supplier
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
Western Blot of Biotin Conjugated Rabbit Anti-Human MIP-3? Antibody. Lane 1: Human MIP-3?. Lane 2: None. Load: 50 ng per lane. Primary antibody: Human MIP-3? antibody at 1:1000 for 60 min at RT. Secondary antibody: Peroxidase conjugated Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 10 kDa, 10 kDa for Human MIP-3?. Other band(s): None.
quantity: 100 µg
price:
to the supplier