Rockland Immunochemicals H3f3c antibody

Histone H3 K9ac Antibody
Rockland Immunochemicals
catalog: 600-401-I72

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, chromatin immunoprecipitation
citations: 1

Western Blot of Rabbit Anti-Histone H3 [ac Lys9] Antibody. Lane 1: HeLa histone preps. Load: 30 µg per lane. Primary antibody: Histone H3 [ac Lys9] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.

Chromatin Immunoprecipitation of Rabbit Anti-Histone H3 [ac Lys9] Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 ug of Histone H3 K9ac was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).
quantity: 50 µg
price:
to the supplier

Histone H3 K4me1/pT3 Antibody
Rockland Immunochemicals
catalog: 600-401-I54

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry

Immunofluorescence Microscopy of Rabbit Anti-Histone H3 [p Thr3, Monomethyl Lys4] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [p Thr3, Monomethyl Lys4] antibody at a 1:100 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 [p Thr3, Monomethyl Lys4] is nuclear and chromosomal. Staining: Histone H3 [p Thr3, Monomethyl Lys4] is expressed in green while the nuclei and aplpha-tubulin were coexpressed with DAPI (blue) and Dylight 550 (red).

Western Blot of Rabbit Anti-Histone H3 [p Thr3, Monomethyl Lys4] Antibody. Lane 1: HeLa histone prep. Lane 2. NIH 3T3 histone prep. Lane 3. C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [p Thr3, Monomethyl Lys4] at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.
quantity: 50 µg
price:
to the supplier

Histone H3 K4me2/pT3 Antibody
Rockland Immunochemicals
catalog: 600-401-I55

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, chromatin immunoprecipitation

Western Blot of Rabbit Anti-Histone H3 [p Thr3, Dimethyl Lys4] Antibody. Lane 1: NIH-3T3 histone prep lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [p Thr3, Dimethyl Lys4] at 1:50 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.

Chromatin Immunoprecipitation of Rabbit Anti-Histone H3 [p Thr3, Dimethyl Lys4] Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of Hisone H3 K4 Me2 pT3 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
quantity: 50 µg
price:
to the supplier

Histone H3 pT3/K4ac Antibody
Rockland Immunochemicals
catalog: 600-401-I56

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, chromatin immunoprecipitation

Western Blot of Rabbit Anti-Histone H3 [p Thr3, ac Lys4] Antibody. Lane 1: HeLa histone prep. 2. NIH-3T3 histone prep. Lane 3: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [p Thr3, ac Lys4] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.

Western Blot of Rabbit Anti-Histone H3 [p Thr3, ac Lys4] Antibody. Lane 1: HeLa histone prep. 2. NIH-3T3 histone prep. Lane 3: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [p Thr3, ac Lys4] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.
quantity: 50 µg
price:
to the supplier

Histone H3 K4me3 Antibody
Rockland Immunochemicals
catalog: 600-401-I59

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, chromatin immunoprecipitation

Rockland Immunochemicals is manufacturing Epi-Plus™ antibody in collaboration with Novus Biologicals.

Western Blot of Rabbit Anti-Histone H3 K4/me3 Antibody. Lane 1: C. elegans embryo nuclear extract. Load: 30 µg per lane. Primary antibody: Histone H3 [Trimethyl Lys4] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.

Chromatin Immunoprecipitaion of Rabbit Anti-Histone H3 K4/me3 Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of Histone H3 K4/me3 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
quantity: 50 µg
price:
to the supplier

Histone H3 K4ac Antibody
Rockland Immunochemicals
catalog: 600-401-I60

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, chromatin immunoprecipitation

Western Blot of Rabbit Anti-Histone H3 [ac Lys4] Antibody. Lane 1: HeLa histone prep. Lane 2: NIH-3T3 prep lysates. Load: 30 µg per lane. Primary antibody: Histone H3 [ac Lys4] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.

Western Blot of Rabbit Anti-Histone H3 [ac Lys4] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [ac Lys4] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15-16 kDa. Other band(s): None.

Chromatin Immunoprecipitation of Rabbit Anti-Histone H3 [ac Lys4] Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of Histone H3 K4ac and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
quantity: 50 µg
price:
to the supplier

Histone H3 K9me1/pT6 Antibody
Rockland Immunochemicals
catalog: 600-401-I64

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, chromatin immunoprecipitation

Western Blot of Rabbit Anti-Histone H3 [Monomethyl Lys9, p Thr6] Antibody. Lane 1: NIH-3T3 Histone prep lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [Monomethyl Lys9, p Thr6] at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.

Chromatin Immunoprecipitation of Histone H3 [p Thr6, Monomethyl Lys9] Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of Histone H3 K9me2pT6 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
quantity: 50 µg
price:
to the supplier

Histone H3 R8me2a Antibody
Rockland Immunochemicals
catalog: 600-401-I67

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, chromatin immunoprecipitation

Western Blot of Rabbit Anti-Histone H3 [Asym-dimethyl Arg8] Antibody. Lane 1: HeLa Histone Prep Lysate. 2. NIH-3T3 Prep Lysate. Lane 3: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [Asym-dimethyl Arg8] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.

Chromatin Immunoprecipitation of Histone H3 [Asym-dimethyl Arg8] Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of Histone H3 R8me2a and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
quantity: 50 µg
price:
to the supplier

Histone H3 K9me1 Antibody
Rockland Immunochemicals
catalog: 600-401-I69

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, chromatin immunoprecipitation

Western Blot of Rabbit Anti-Histone H3 [Monomethyl Lys9] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [Monomethyl Lys9] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.

Chromatin Immunoprecipitation of Histone H3 [Monomethyl Lys9] Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of Histone H3 K9Me1 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
quantity: 50 µg
price:
to the supplier

Histone H3 K9ac/K14ac Antibody
Rockland Immunochemicals
catalog: 600-401-I73

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, chromatin immunoprecipitation

Western Blot of Rabbit Anti-Histone H3 [ac Lys9, ac Lys14] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [ac Lys9, ac Lys14] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.

Dot Blot of Rabbit Histone H3 [K9ac, K14ac] Antibody. Lane 1: K9-K14. Lane 2: K9-KAc-K14-Kac. Lane 3: K9-K14-Kac. Lane 4: K9-Ac-K14. Load: 0.3, 1, and 3 µg of peptide. Primary antibody: Histone H3 [ac Lys9, ac Lys14] antibody for 45 min at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO A overnight at 4°C.
quantity: 50 µg
price:
to the supplier

Histone H3 R17me2a Antibody
Rockland Immunochemicals
catalog: 600-401-I77

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, chromatin immunoprecipitation

Western Blot of Rabbit Anti-Histone H3 [Asym-dimethyl Arg17] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [Asym-dimethyl Arg17] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.

Chromatin Immunoprecipitation of Rabbit Anti Histone H3 [Asym-dimethyl Arg17] Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 ug of Histone H3R17me2a was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).
quantity: 50 µg
price:
to the supplier

Histone H3 K18me1 Antibody
Rockland Immunochemicals
catalog: 600-401-I78

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, chromatin immunoprecipitation

Western Blot of Rabbit Anti-Histone H3 [Monomethyl Lys18] Antibody. Lane 1: NIH-3T3 histone prep cell lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [Monomethyl Lys18] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.

Western Blot of Rabbit Anti-Histone H3 [Monomethyl Lys18] Antibody. Lane 1: HeLa histone prep lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [Monomethyl Lys18] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.
quantity: 50 µg
price:
to the supplier

Histone H3 K18me2 Antibody
Rockland Immunochemicals
catalog: 600-401-I79

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, chromatin immunoprecipitation

Immunofluorescence of Rabbit Anti-Histone H3 [Dimethyl Lys18] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [Dimethyl Lys18] antibody at a 1:500 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 [Dimethyl Lys18] is nuclear and chromosomal. Staining: Histone H3 [Dimethyl Lys18] is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 594 (red).

Western Blot of Rabbit Anti-Histone H3 [Dimethyl Lys18] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [Dimethyl Lys18] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.

Chromatin Immunoprecipitation of Rabbit Anti-Histone H3 [Dimethyl Lys18] Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of Histone H3 K18me2 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
quantity: 50 µg
price:
to the supplier

Histone H3 K18me3 Antibody
Rockland Immunochemicals
catalog: 600-401-I80

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, chromatin immunoprecipitation

Western Blot of Rabbit Anti-Histone H3 [Trimethyl Lys18] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [Trimethyl Lys18] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.

Western Blot of Rabbit Anti-Histone H3 [Trimethyl Lys18] Antibody. Lane 1: NIH-3T3 histone preps. Load: 30 µg per lane. Primary antibody: Histone H3 [Trimethyl Lys18] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.
quantity: 50 µg
price:
to the supplier

Histone H3 K18ac Antibody
Rockland Immunochemicals
catalog: 600-401-I81

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, chromatin immunoprecipitation

Western Blot of Rabbit Anti-Histone H3 [ac Lys18] Antibody. Lane 1: HeLa histone prep. Lane 2: NIH-3T3 histone prep. Lane 3: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [ac Lys18] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.

Chromatin Immunoprecipitation Rabbit Anti-Histone H3 [ac Lys18] Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of Histone H3K18ac and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
quantity: 50 µg
price:
to the supplier

Histone H3 K23ac Antibody
Rockland Immunochemicals
catalog: 600-401-I82

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, chromatin immunoprecipitation

Western Blot of Rabbit Anti-Histone H3 [ac Lys23] Antibody. Lane 1: NIH-3T3 histone prep lysate. Lane 2: HeLa histone prep lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [ac Lys23] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.

Western Blot of Rabbit Anti-Histone H3 [ac Lys23] Antibody. Lane 1: NIH-3T3 histone prep lysate. Lane 2: HeLa histone prep lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [ac Lys23] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.
quantity: 50 µg
price:
to the supplier

Histone H3 pS28 Antibody
Rockland Immunochemicals
catalog: 600-401-I83

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, chromatin immunoprecipitation

Dot Blot of Rabbit Histone H3 [p Ser28] Antibody. Lane 1: pS28. Lane 2: S28 unmodified. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 [p Ser28] antibody at 1:1000 for 45 min at 4°C. Secondary antibody: Dylight™488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.

Western Blot of Rabbit Anti-Histone H3 [p Ser28] Antibody. Lane 1: NIH-3T3 histone prep. Load: 30 µg per lane. Primary antibody: Histone H3 [p Ser28] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.

Dot Blot of Rabbit Histone H3 [p Ser28] Antibody. Lane 1: pS28. Lane 2: S28 unmodified. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 [p Ser28] antibody at 1:1000 for 45 min at 4°C. Secondary antibody: Dylight™488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.
quantity: 50 µg
price:
to the supplier

Histone H3 K27me3/pS28 Antibody
Rockland Immunochemicals
catalog: 600-401-I84

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, chromatin immunoprecipitation

Immunofluorescence of Rabbit Anti-Histone H3 [p Ser28, Trimethyl Lys27] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [p Ser28, Trimethyl Lys27] antibody at a 1:2000 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 [p Ser28, Trimethyl Lys27] is nuclear and chromosomal. Staining: Histone H3 [p Ser28, Trimethyl Lys27] is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red).

Immunofluorescence of Rabbit Anti-Histone H3 [p Ser28, Trimethyl Lys27] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [p Ser28, Trimethyl Lys27] antibody at a 1:2000 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 [p Ser28, Trimethyl Lys27] is nuclear and chromosomal. Staining: Histone H3 [p Ser28, Trimethyl Lys27] is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red).
quantity: 50 µg
price:
to the supplier

Histone H3 R2me2s/K4me2 Antibody
Rockland Immunochemicals
catalog: 600-401-I85

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, chromatin immunoprecipitation

Western Blot of Rabbit Anti-Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4] Antibody. Lane 1: C. elegans embryo cell lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.

Chromatin Immunoprecipitation of Rabbit Anti-Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4] Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of Histone H3 R2me2s-K4me2 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
quantity: 50 µg
price:
to the supplier

Histone H3 K36me2 Antibody
Rockland Immunochemicals
catalog: 600-401-I87

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, chromatin immunoprecipitation

Western Blot of Rabbit Anti-Histone H3 [Dimethyl Lys36] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [Dimethyl Lys36] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.

Chromatin Immunoprecipitation of Rabbit Anti-Histone H3 [Dimethyl Lys36] Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of Histone H3 K36me2 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
quantity: 50 µg
price:
to the supplier

Histone H3 K36me3 Antibody
Rockland Immunochemicals
catalog: 600-401-I88

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry

Western Blot of Rabbit Anti-Histone H3 [Trimethyl Lys36] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [Trimethyl Lys36] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.

Dot Blot of Rabbit Histone H3 [Trimethyl Lys36] Antibody. Lane 1: K36. Lane 2: K36Me1. Lane 3: K36Me2. Lane 4: K36Me3. Lane 5: K36Ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 [Trimethyl Lys36] antibody at 0.5 µg/ml for 45 min at 4°C. Secondary antibody: Dylight™488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.
quantity: 50 µg
price:
to the supplier

Histone H3 K37me1 Antibody
Rockland Immunochemicals
catalog: 600-401-I90

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, chromatin immunoprecipitation

Dot Blot of Rabbit Histone H3 [Monomethyl Lys37] Antibody. Lane 1: K37. Lane 2: K37Ac. Lane 3: K37Me1. Lane 4: K37Me2. Lane 5: K37Me3. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 [Monomethyl Lys37] antibody at 1:1000 for 45 min at 4°C. Secondary antibody: Dylight™488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.

Dot Blot of Rabbit Histone H3 [Monomethyl Lys37] Antibody. Lane 1: K37. Lane 2: K37Ac. Lane 3: K37Me1. Lane 4: K37Me2. Lane 5: K37Me3. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 [Monomethyl Lys37] antibody at 1:1000 for 45 min at 4°C. Secondary antibody: Dylight™488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.
quantity: 50 µg
price:
to the supplier

Histone H3 K37me2 Antibody
Rockland Immunochemicals
catalog: 600-401-I91

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, chromatin immunoprecipitation

Immunofluorescence of Rabbit Anti-Histone H3 [Dimethyl Lys37] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [Dimethyl Lys37] antibody at a 1:100 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 [Dimethyl Lys37] is nuclear and chromosomal. Staining: Histone H3 [Dimethyl Lys37] is expressed in green, nuclei are counterstained with DAPI (blue).

Western Blot of Rabbit Anti-Histone H3 [Dimethyl Lys37] Antibody. Lane 1: NIH-3T3 histone prep. Lane 2: C. elegans embryo. Lane 3: HeLa histone prep. Load: 30 µg per lane. Primary antibody: Histone H3 [Dimethyl Lys37] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.
quantity: 50 µg
price:
to the supplier

Histone H3 K56me1 Antibody
Rockland Immunochemicals
catalog: 600-401-I93

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, chromatin immunoprecipitation

Immunofluorescence of Rabbit Anti-Histone H3 [Dimethyl Lys37] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [Dimethyl Lys37] antibody at a 1:100 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 [Dimethyl Lys37] is nuclear and chromosomal. Staining: Histone H3 [Dimethyl Lys37] is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red).

Western Blot of Rabbit Anti-Histone H3 [Monomethyl Lys56] Antibody. Lane 1: NIH-3T3 histone prep lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [Monomethyl Lys56] at 1 µg/ml for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.

Dot Blot of Rabbit Histone H3 [Monomethyl Lys56] Antibody. Lane 1: K56Ac. Lane 2: K56Me1. Lane 3: K56Me2. Lane 4: K56Me3. Lane 5: K56 unmodified. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 [Monomethyl Lys56] antibody at 1:20-1:100 for 45 min at 4°C. Secondary antibody: Dylight™488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.
quantity: 50 µg
price:
to the supplier

Histone H3 K27ac Antibody
Rockland Immunochemicals
catalog: 600-401-K00

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunoprecipitation

quantity: 50 µg
price:
to the supplier

Histone H3 K9-Ac/pS10 (RABBIT) Antibody
Rockland Immunochemicals
catalog: 600-401-P08

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

quantity: 50 µg
price:
to the supplier

Histone H3 pT3/R2-Me2s Antibody
Rockland Immunochemicals
catalog: 600-401-P14

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

quantity: 50 µg
price:
to the supplier