domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
citations: 1
reactivity: human
application: western blot, ELISA
citations: 1
Western blot using Rockland's affinity purified anti-p90 RSK1 pS732 antibody shows detection of a band ~90 kDa in size corresponding to phosphorylated p90 RSK1 (arrowhead) in EGF stimulated (lane 2) HEK293T cell lysates prepared from cells grown in the absence of serum for 12 h. No staining is observed in similarly prepared lysates derived from unstimulated (control) cells (lane 1). After transfer, the membrane was blocked overnight followed by reaction with the primary antibody at a 1:1,000 dilution. Detection occurred using a peroxidase conjugated secondary antibody and ECL. Personal Communication. Kuldeep Patel, Loyola University Medical Center, Maywood, IL.
quantity: 100 µg
price:
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunoprecipitation
reactivity: human, mouse
application: western blot, ELISA, immunoprecipitation
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
reactivity: human
application: western blot, ELISA
Western Blot of Rabbit anti-p90 RSK1 antibody. Lane 1: unstimulated HEK293T cell lysates. Lane 2: EGF stimulated HEK293T cell lysates. Load: 35 µg per lane. Primary antibody: p90 RSK1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: peroxidase conjugated secondary antibody and ECL. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 90 kDa, ~90 kDa for p90 RSK1. Other band(s): none.
quantity: 100 µg
price:
to the supplier
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
reactivity: human
application: western blot, ELISA