Tryptophan Hydroxylase 2 phospho S19 Antibody | Rockland Immunochemicals 612-401-E48 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Tryptophan Hydroxylase 2 phospho S19 Antibody

catalog :

612-401-E48

quantity :

100 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

rat

application :

western blot

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

612-401-E48

Name :

Anti-Tryptophan Hydroxylase 2 pS19 (Rabbit) Antibody - 612-401-E48

Display Name :

Tryptophan Hydroxylase 2 phospho S19 Antibody

Application Note :

Anti-Trytophan Hydroxylase2 pS19 (Rabbit) antibody is suitable for use in Western Blotting. Expect a band of approximately 55 kDa in size corresponding to tryptophan hydroxylase protein phosphorylated at Ser 19 in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.

Buffer :

0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Clonality :

Polyclonal

Concentration Value :

Titrated value sufficient to run approximately 10 mini blots.

Conjugation :

(None)

Size :

100 µL

Default Unit :

Western Blot Dilution :

1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

TPH2

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-Tryptophan Hydroxylase 2 pS19 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser19 of Tryptophan Hydroxylase.

Packing Type :

Dry Ice

Physical State :

Liquid

Purity and Specificity :

Anti-Tryptophan Hydroxylase 2 is directed against rat Tryptophan Hydroxylase phosphorylated at Ser19. Tryptophan Hydroxylase 2 pS19 antibodies are prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Reactivity is expected from the following species based on 100% sequence homology: bovine, mouse and zebra fish.

Species Reactivity :

rat

Stabilizer :

0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol

Storage :

Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Synonyms :

Tryptophan 5-hydroxylase 2, Neuronal tryptophan hydroxylase, Tryptophan 5-monooxygenase 2, TPH

Target Species :

Rat

Background :

Tryptophan Hydroxylase 2 pS19 antibody detects TPH2 protein. Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II). CaM K II phosphorylates Ser19 which lies within the regulatory domain of TPH2. Anti-TPH2pS19 antibody is ideal for investigators involved in Cell Signaling and Neuroscience research.

Immunogen Type :

Peptide

Low Endotoxin :

Post Translational Modification :

Phosphorylation

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q8CGU9

Primary Image Name :

Tryptophan Hydroxylase phospho S19 Antibody - Western Blot

more info or order :

Rockland Immunochemicals product webpage