Potassium Chloride Cotransporter (KCC2) phospho S940 Antibody | Rockland Immunochemicals 612-401-E15 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Potassium Chloride Cotransporter (KCC2) phospho S940 Antibody

catalog :

612-401-E15

quantity :

100 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

rat

application :

western blot

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

612-401-E15

Name :

Anti-Potassium Chloride Cotransporter (KCC2) pS940 (Rabbit) Antibody - 612-401-E15

Display Name :

Potassium Chloride Cotransporter (KCC2) phospho S940 Antibody

Application Note :

Anti-Potassium Chloride Cotransporter (KCC2) Antibody is suitable for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 135 kDa in size corresponding to KCC2 protein phosphorylated at Ser940 in the appropriate cell lysate or extract.

Buffer :

0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Clonality :

Polyclonal

Concentration Value :

Sufficient for 10 mini blots (total final volume 100 ml at 1:1000 dilution)

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µL

Default Unit :

Western Blot Dilution :

1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

Slc12a5

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-Potassium Chloride Cotransporter (KCC2) pS940 Antibody was produced by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser940.

Packing Type :

Dry Ice

Physical State :

Liquid

Purity and Specificity :

Anti-Potassium Chloride Cotransporter (KCC2) pS940 antibody is directed against phosphorylated rat KCC2 protein. The antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Immunolabeling of the KCC2 protein band is blocked by the phospho-peptide used as antigen but not by the corresponding dephosphopeptide. Reactivity is expected fromthe following species based on 100% sequence homology: bovine, canine, human, mouse and non-human primate.

Species Reactivity :

rat

Stabilizer :

0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol

Storage :

Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Synonyms :

Solute carrier family 12 member 5, Electroneutral potassium-chloride cotransporter 2, Furosemide-sensitive K-Cl cotransporter, K-Cl cotransporter 2, rKCC2, Neuronal K-Cl cotransporter, Kcc2

Target Species :

Rat

Background :

Potassium Chloride Cotransporter pS940 Antibody detects Potassium Chloride Cotransporter pS940 protein. KCC2 is widely thought to be expressed exclusively in neurons where it is responsible for maintaining low intracellular chloride concentration to drive hyperpolarizing post-synaptic responses to the inhibitory neurotransmitters GABA and glycine. Serine 940 on KCC2 has been shown to be phosphorylated by PKC and has further been demonstrated to be the major site for PKC-dependent phosphorylation for full length KCC2 molecules when expressed in HEK-293 cells as phosphorylation of Ser940 increased the cell surface stability of KCC2 in this system by decreasing it’s rate of internalization from the plasma membrane. Anti-Potassium Chloride Cotransporter (KCC2) pS940 Antibody is ideal for investigators involved in Neuroscience and Cell Signaling research.

Immunogen Type :

Peptide

Low Endotoxin :

Post Translational Modification :

Phosphorylation

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q63633

Primary Image Name :

Western blot of Anti-Potassium Chloride Cotransporter (KCC2) pS940 (Rabbit) Antibody - 612-401-E15

more info or order :

Rockland Immunochemicals product webpage