MEK5 phospho S311/phospho T315 Antibody | Rockland Immunochemicals 612-401-D74 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

MEK5 phospho S311/phospho T315 Antibody

catalog :

612-401-D74

quantity :

100 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

rat

application :

western blot

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

612-401-D74

Name :

Anti-MEK5 pS311/pT315 (Rabbit) Antibody - 612-401-D74

Display Name :

MEK5 phospho S311/phospho T315 Antibody

Application Note :

Anti-MEK5 pS311/pT315 (Rabbit) antibody is suitable for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 49 kDa in size corresponding to MEK5 protein phosphorylated at Ser311/Thr315 in the appropriate cell lysate or extract.

Buffer :

0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Clonality :

Polyclonal

Concentration Value :

Titrated value sufficient to run approximately 10 mini blots.

Conjugation :

(None)

Size :

100 µL

Default Unit :

Western Blot Dilution :

1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

Map2k5

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-MEK5 pS311/pT315 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser311/Thr315 conjugated to KLH.

Packing Type :

Dry Ice

Physical State :

Liquid

Purity and Specificity :

Anti-MEK5 pS311/pT315 antibody is directed against rat MEK5. MEK5 antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Immunolabeling of the MEK5 band is blocked by preadsorption with the phospho-peptide used as antigen but not be the corresponding dephospho-peptide. Expect reactivity with the following species based on sequence homology: bovine, canine, chicken, human, mouse, non-human primates, Xenopus and zebra fish. Cross reactivity with MEK5 pS311/pT315 from other species has not been determined.

Species Reactivity :

rat

Stabilizer :

0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol

Storage :

Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Synonyms :

Dual specificity mitogen-activated protein kinase kinase 5, MAP kinase kinase 5, MAPKK 5, MAPK/ERK kinase 5

Target Species :

Rat

Background :

MEK5 pS311/pT315 antibody detects MEK5 that is phosphorylated at Ser311 and Threonine315. MEK5 (also known as MKK5) is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family. MEK5 has been shown to specifically activate ERK5 whereas MEK5 itself is regulated by MEKK3. An important link between MEK5 and metastatic prostrate cancer has been demonstrated. Dual phosphorylation of Ser311 and Thr315 have been implicated in cell proliferation. Therefore, MEK5 pS311/pT315 antibody is ideal for investigators involved in Cell Signaling, Cell Migration and Proliferation and, more generally in Cancer Research.

Immunogen Type :

Peptide

Low Endotoxin :

Post Translational Modification :

Phosphorylation

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q62862

Primary Image Name :

Western Blot of Anti-MEK5 pS311/pT315 (Rabbit) Antibody - 612-401-D74

more info or order :

Rockland Immunochemicals product webpage