product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
MARCKS phospho S152/phospho S156 Antibody
catalog :
612-401-D70
quantity :
100 µL
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot
more info or order :
product information
Catalog Number :
612-401-D70
Name :
Anti-MARCKS pS152/pS156 (Rabbit) Antibody - 612-401-D70
Display Name :
MARCKS phospho S152/phospho S156 Antibody
Application Note :
Anti-MARCKS pS152/pS156 (Rabbit) antibody is suitable for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 87 kDa in size corresponding to MARCKS protein phosphorylated at Ser 152/156 in the appropriate cell lysate or extract.
Buffer :
0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Clonality :
Polyclonal
Concentration Value :
Titrated value sufficient to run approximately 10 mini blots.
Conjugation :
(None)
Size :
100 µL
Default Unit :
ul
Western Blot Dilution :
1:1000
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG
Gene Name :
Marcks
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Anti-MARCKS pS152/pS156 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser 152/156 conjugated to KLH.
Packing Type :
Dry Ice
Physical State :
Liquid
Purity and Specificity :
Anti-MARCKS pS152/pS156 antibody is directed against MARKS doubly phosphoylated at Ser152 and Ser156. MARCKS antibody are affinity purified from monospecific antiserum by immunoaffinity purification. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide. The immunolabeling is completely eliminated by lambda-phosphatase. Expect reactivity with the following species based on sequence homology: bovine, chicken, human, mouse, Xenopus and zebra fish. Cross reactivity with MARCKS pS152/pS156 from other species has not been determined.
Species Reactivity :
rat, mouse
Stabilizer :
0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol
Storage :
Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Synonyms :
Myristoylated alanine-rich C-kinase substrate, Protein kinase C substrate 80 kDa protein
Target Species :
Rat
Background :
MARCKS pS152/pS156 antibody detects MARKS when phosphoylated at both Ser152 and Ser156 Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC). The phosphorylation of Ser152/156 can be used as a measure of PKC activation although these sites are also phosphorylated by PRK1 MARCKS is a member of a family of calmodulin binding proteins and phosphorylation of Ser 152/156 modulates the binding of MARCKS to calmodulin. Therefore, phosphorylated MARCKS antibody is ideal for investigators involved in Cell Signaling, Cell migration and Cancer Research.
Immunogen Type :
Peptide
Low Endotoxin :
No
Post Translational Modification :
Phosphorylation
Sample Size :
No
Target Domain of Reactivity :
Internal
Application Text :
Western Blot,
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
UniProt :
P30009
Primary Image Name :
Western Blot of Anti-MARCKS pS152/pS156 (Rabbit) Antibody - 612-401-D70
more info or order :
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.