product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
14-3-3 Antibody
catalog :
612-401-D01
quantity :
100 µL
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
rat
application :
western blot
more info or order :
product information
Catalog Number :
612-401-D01
Name :
Anti-14-3-3 (Rabbit) Antibody - 612-401-D01
Display Name :
14-3-3 Antibody
Application Note :
14-3-3 antibody is suitable for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 29 kDa in size corresponding to 14-3-3 protein in the appropriate cell lysate or extract.
Buffer :
0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Clonality :
Polyclonal
Concentration Value :
Titrated value sufficient to run approximately 10 mini blots.
Conjugation :
(None)
Size :
100 µL
Default Unit :
ul
Western Blot Dilution :
1:1000
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG
Gene Name :
Ywhab
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Anti-14-3-3 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues from the C-terminal region.
Packing Type :
Dry Ice
Physical State :
Liquid
Purity and Specificity :
Anti-14-3-3 Antibody is directed against rat 14-3-3 protein. The antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Immunolabeling of the 14-3-3 protein band is completely blocked by pre-adsorption of the antibody with the peptide that was used to generate the antibody. Reactivity is expected from the following species based on 100% sequence homology: bovine, canine, chicken, human, mouse, non-human primates, Xenopus and zebra fish.
Species Reactivity :
rat
Stabilizer :
0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol
Storage :
Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Synonyms :
14-3-3 protein beta/alpha, Prepronerve growth factor RNH-1, Protein kinase C inhibitor protein 1, KCIP-1, 14-3-3 protein beta/alpha N-terminally processed, Ywhab
Target Species :
Rat
Background :
14-3-3 Antibody detects 14-3-3 protein. 14-3-3 is member of a family of highly conserved proteins that appear to have multiple roles in cell signaling. 14-3-3 is abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders. 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms. The 14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking. Anti-14-3-3 antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.
Immunogen Type :
Peptide
Low Endotoxin :
No
Sample Size :
No
Target Domain of Reactivity :
C-Terminus
Application Text :
Western Blot,
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
UniProt :
P35213
NCBI :
NP_062250.1
Primary Image Name :
Western Blot of Anti-14-3-3 Protein (Rabbit) Antibody - 612-401-D01
more info or order :
company information

Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.
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