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company name :
Rockland Immunochemicals
product type :
secondary antibody
product name :
Rat IgG IgA IgM (H&L) Antibody Fluorescein Conjugated
catalog :
612-102-130
quantity :
2 mg
more info or order :
Rockland Immunochemicals product webpage
product information
Catalog Number :
612-102-130
Name :
Anti-Rat IgG IgA IgM (H&L) (GOAT) Antibody Fluorescein Conjugated - 612-102-130
Display Name :
Rat IgG IgA IgM (H&L) Antibody Fluorescein Conjugated
Application Note :
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
2.0 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
FITC
Size :
2 mg
Default Unit :
mg
FLISA Dilution :
1:10,000 - 1:50,000
Flow Cytometry Dilution :
1:500 - 1:2,500
Fluorochrome Protein Value :
3.5
IF Microscopy Dilution :
1:1,000 - 1:5,000
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG
Host Animal :
Goat
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Rat IgG, IgA and IgM whole molecules
Packing Type :
Ambient
Physical State :
Lyophilized
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rat IgG, Rat IgA and Rat IgM.  This reagent is suitable for the detection of all Rat isotypes and chain combinations.
Reconstitution Buffer :
Restore with deionized water (or equivalent)
Reconstitution Volume :
1.0 mL
Stabilizer :
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Storage :
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Synonyms :
goat anti-Rat IgG IgA IgM Antibody fluorescein conjugation, Goat Anti-rat IgGAM FITC conjugated antibody
Target Species :
Rat
Background :
Anti-Rat IgG IgA IgM (H&L) Antibody generated in goat detects rat (heavy and light chain) immunoglobulin G, A, and M. Immunoglobulin G binds to antigens and can neutralize or opsinize targets, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunoglobulin molecules linked through their F(c) domain by the J chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rat IgG IgA IgM Antibody is conjugated to Fluorescein.
Low Endotoxin :
No
Sample Size :
No
Secondary Antibody Specificity :
IgG IgA IgM
Application Text :
FLISA,Flow Cytometry,IF Microscopy,
Other :
FLOW CYTOMETRY 1:500 - 1:2,500
Category :
Secondary Antibodies
Conjugation Name :
Fluorescein (FITC)
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.