product summary
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company name :
Rockland Immunochemicals
product type :
secondary antibody
product name :
Rabbit IgG (H&L) Antibody DyLight™ 800 Conjugated
catalog :
611-145-002
quantity :
100 µg
more info or order :
Rockland Immunochemicals product webpage
citations: 13
Reference
Takahashi H, Ranjan A, Chen S, Suzuki H, Shibata M, Hirose T, et al. The role of Mediator and Little Elongation Complex in transcription termination. Nat Commun. 2020;11:1063 pubmed publisher
Mitra S, Bodor D, David A, Abdul Zani I, Mata J, Neumann B, et al. Genetic screening identifies a SUMO protease dynamically maintaining centromeric chromatin. Nat Commun. 2020;11:501 pubmed publisher
Cao Z, Xue J, Cheng Y, Wang J, Liu Y, Li H, et al. MDM2 promotes genome instability by ubiquitinating the transcription factor HBP1. Oncogene. 2019;38:4835-4855 pubmed publisher
Myles I, Anderson E, Earland N, Zarember K, Sastalla I, Williams K, et al. TNF overproduction impairs epithelial staphylococcal response in hyper IgE syndrome. J Clin Invest. 2018;128:3595-3604 pubmed publisher
Zhang G, Kang Y, Zhou C, Cui R, Jia M, Hu S, et al. Amelioratory Effects of Testosterone Propionate on Age-related Renal Fibrosis via Suppression of TGF-?1/Smad Signaling and Activation of Nrf2-ARE Signaling. Sci Rep. 2018;8:10726 pubmed publisher
Lin X, Cui M, Xu D, Hong D, Xia Y, Xu C, et al. Liver-specific deletion of Eva1a/Tmem166 aggravates acute liver injury by impairing autophagy. Cell Death Dis. 2018;9:768 pubmed publisher
Heubl M, Zhang J, Pressey J, Al Awabdh S, Renner M, Gomez Castro F, et al. GABAA receptor dependent synaptic inhibition rapidly tunes KCC2 activity via the Cl--sensitive WNK1 kinase. Nat Commun. 2017;8:1776 pubmed publisher
Xia D, Ji W, Xu C, Lin X, Wang X, Xia Y, et al. Knockout of MARCH2 inhibits the growth of HCT116 colon cancer cells by inducing endoplasmic reticulum stress. Cell Death Dis. 2017;8:e2957 pubmed publisher
Wang X, Gregory Evans K, Wasan K, Sivak O, Shan X, Gregory Evans C. Efficacy of Postnatal In Vivo Nonsense Suppression Therapy in a Pax6 Mouse Model of Aniridia. Mol Ther Nucleic Acids. 2017;7:417-428 pubmed publisher
Li G, Xu C, Lin X, Qu L, Xia D, Hongdu B, et al. Deletion of Pdcd5 in mice led to the deficiency of placenta development and embryonic lethality. Cell Death Dis. 2017;8:e2811 pubmed publisher
Wang S, Cao Z, Xue J, Li H, Jiang W, Cheng Y, et al. A positive feedback loop between Pim-1 kinase and HBP1 transcription factor contributes to hydrogen peroxide-induced premature senescence and apoptosis. J Biol Chem. 2017;292:8207-8222 pubmed publisher
Chen Y, Pan K, Wang P, Cao Z, Wang W, Wang S, et al. HBP1-mediated Regulation of p21 Protein through the Mdm2/p53 and TCF4/EZH2 Pathways and Its Impact on Cell Senescence and Tumorigenesis. J Biol Chem. 2016;291:12688-705 pubmed publisher
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product information
Catalog Number :
611-145-002
Name :
Anti-RABBIT IgG (H&L) (GOAT) Antibody DyLight™ 800 Conjugated - 611-145-002
Display Name :
Rabbit IgG (H&L) Antibody DyLight™ 800 Conjugated
Application Note :
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
1.0 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
DL800
Size :
100 µg
Default Unit :
µg
FLISA Dilution :
>1:20,000
Fluorochrome Protein Value :
1.7
IF Microscopy Dilution :
>1:5,000
Western Blot Dilution :
>1:10,000
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG
Host Animal :
Goat
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Rabbit IgG whole molecule
Packing Type :
Ambient
Physical State :
Lyophilized
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. This antibody will react with heavy chains of Rabbit IgG and with light chains of most Rabbit immunoglobulins.
Reconstitution Buffer :
Restore with deionized water (or equivalent)
Reconstitution Volume :
100 µL
Species Reactivity :
Rabbit
Stabilizer :
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Storage :
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Synonyms :
Goat anti-Rabbit IgG Antibody DyLight™800 Conjugation, Goat anti-Rabbit IgG DyLight™ 800 Conjugated Antibody
Target Species :
Rabbit
Background :
Anti-Rabbit IgG Antibody DyLight™800 generated in goat detects rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rabbit IgG (H&L) is conjugated to DyLight™800.
Low Endotoxin :
No
Sample Size :
No
Secondary Antibody Specificity :
IgG (H&L)
Application Text :
FLISA,IF Microscopy,Western Blot,
Other :
User Optimized
Category :
Secondary Antibodies
Conjugation Name :
DyLight™ 800
Primary Image Name :
Dylight conjugated antibody - Western Blot
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.