Rabbit IgG (H&L) Antibody Phycoerythrin conjugated Pre-Adsorbed | Rockland Immunochemicals 611-108-122 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Rabbit IgG (H&L) Antibody Phycoerythrin conjugated Pre-Adsorbed

catalog :

611-108-122

quantity :

1 mL

clonality :

polyclonal

host :

domestic goat

conjugate :

RPE

application :

flow cytometry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

611-108-122

Name :

Anti-RABBIT IgG [H&L] (GOAT) Antibody Phycoerythrin conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) - 611-108-122

Display Name :

Rabbit IgG (H&L) Antibody Phycoerythrin conjugated Pre-Adsorbed

Application Note :

Anti-Rabbit IgG (H&L) Phycoerythrin Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

0.5 mg/mL

Concentration Definition :

by absorbance = 82.0 at 565 nm

Conjugation :

RPE

Size :

1 mL

Default Unit :

Flow Cytometry Dilution :

1:100 - 1:250

IF Microscopy Dilution :

1:100 - 1:250

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Host Animal :

Goat

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-Rabbit IgG was produced by repeated immunization with rabbit IgG whole molecule in goat.

Packing Type :

Ambient

Physical State :

Lyophilized

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Rabbit IgG (H&L) Antibody Phycoerythrin conjugated Pre-Adsorbed was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, conjugation to phycoerythrin and subsequent purification, and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.

Reconstitution Buffer :

Restore with deionized water (or equivalent)

Reconstitution Volume :

1.0 mL

Species Reactivity :

Rabbit

Stabilizer :

10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Storage :

Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.

Synonyms :

goat anti-Rabbit IgG Antibody, Gt-a-Rabbit Phycoerythrin conjugated, Rabbit IgG (H&L) Antibody in goat, Goat IgG (H&L) Phycoerythrin conjugated Secondary Antibody.

Target Species :

Rabbit

Background :

Anti-Rabbit IgG (H&L) phycoerythrin conjugated antibody generated in goat detects specifically rabbit IgG. This secondary phycoerythrin conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, microscopy and FACS analysis.

Immunogen Type :

Native Protein

Low Endotoxin :

Sample Size :

Secondary Antibody Specificity :

IgG (H&L)

Application Text :

Flow Cytometry,IF Microscopy,

Other :

FLOW 1:100 - 1:250

Category :

Secondary Antibodies

Conjugation Name :

R-Phycoerythrin (RPE)

Primary Image Name :

WB - Rabbit IgG (H&L) Antibody Phycoerythrin conjugated Pre-Adsorbed

more info or order :

Rockland Immunochemicals product webpage