product summary
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company name :
Rockland Immunochemicals
product type :
secondary antibody
product name :
Rabbit IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-adsorbed
catalog :
611-103-122
quantity :
1
price :
290.00 USD
more info or order :
Rockland Immunochemicals product webpage
citations: 31
Reference
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Na Z, Luo Y, Cui D, Khitun A, Smelyansky S, Loria J, et al. Phosphorylation of a Human Microprotein Promotes Dissociation of Biomolecular Condensates. J Am Chem Soc. 2021;143:12675-12687 pubmed publisher
Luo Y, Schofield J, Na Z, Hann T, Simon M, Slavoff S. Discovery of cellular substrates of human RNA-decapping enzyme DCP2 using a stapled bicyclic peptide inhibitor. Cell Chem Biol. 2020;: pubmed publisher
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Kim M, Wu M, Zhang Y, Yang J, Chang C. TET2 directs mammary luminal cell differentiation and endocrine response. Nat Commun. 2020;11:4642 pubmed publisher
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product information
Catalog Number :
611-103-122
Name :
Goat Anti-Rabbit IgG (H&L) Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) - 611-103-122
Display Name :
Rabbit IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-adsorbed
Application Note :
Anti-Rabbit secondary antibody conjugated to horseradish peroxidase (HRP) generated in goat detects specifically rabbit IgG. This anti-rabbit HRP mx10 secondary antibody has been tested in ELISA, Dot Blot, and Western Blot. Although not tested, this product could be useful for Sandwich ELISA, titration assays, immunoprecipitation, immunohistochemistry as well as other HRP antibody based assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
1.0 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
HRP
Size :
1
Default Unit :
mg
ELISA Dilution :
1:20,000 - 1:100,000
Immunohistochemistry Dilution :
1:1,000 - 1:5,000
Western Blot Dilution :
1:2,000 - 1:10,000
Expiration :
Expiration date is one (1) year from date of receipt.
Format :
IgG
Host Animal :
Goat
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Rabbit IgG whole molecule
Packing Type :
Ambient
Physical State :
Lyophilized
Preservative :
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Purity and Specificity :
Conjugated Second Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
Reconstitution Buffer :
Restore with deionized water (or equivalent)
Reconstitution Volume :
1.0 mL
Species Reactivity :
Rabbit
Tested Applications :
Dot Blot; ELISA; WB
Suggested Applications :
IHC
Stabilizer :
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Storage :
Store secondary antibody at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Synonyms :
goat anti-rabbit hrp, HRP conjugated secondary antibody, goat anti-rabbit secondary antibody, goat anti-rabbit IgG peroxidase conjugated secondary
Target Species :
Rabbit
Background :
Goat anti-Rabbit IgG (H&L) Antibody Conjugated to Horseradish Peroxidase (HRP) is designed for chemiluminescent detection. Secondary antibodies bind to the primary antibody to assist in detection, sorting and purification of target antigens. To enable detection, the secondary antibody must have specificity for the antibody species and isotype of the primary antibody being used and generally is conjugated. This purified secondary antibody has been validated and optimized yielding good sensitivity and reproducible results with Rockland's primary antibodies. This anti-rabbit IgG antibody has been crossed adsorbed to show no reaction against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
Low Endotoxin :
No
Sample Size :
No
Secondary Antibody Specificity :
IgG (H&L)
Application Text :
ELISA,Immunohistochemistry,Western Blot,
Other :
User Optimized
Category :
Secondary Antibodies
Conjugation Name :
Peroxidase (HRP)
2025 Price :
290.00 USD
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.