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company name :
Rockland Immunochemicals
product type :
secondary antibody
product name :
Mouse IgG2a (Gamma 2a chain) Antibody Alkaline Phosphatase Conjugated
catalog :
610-4541
quantity :
1 mg
more info or order :
Rockland Immunochemicals product webpage
product information
Catalog Number :
610-4541
Name :
Anti-MOUSE IgG2a (Gamma 2a chain) (RABBIT) Antibody Alkaline Phosphatase Conjugated - 610-4541
Display Name :
Mouse IgG2a (Gamma 2a chain) Antibody Alkaline Phosphatase Conjugated
Application Note :
This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:5,000 is suggested for this product.
Buffer :
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Clonality :
Polyclonal
Concentration Value :
1.0 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
ALP
Size :
1 mg
Default Unit :
mg
ELISA Dilution :
1:2,000 - 1:10,000
Immunohistochemistry Dilution :
1:200 - 1:1,000
Western Blot Dilution :
1:500 - 1:2,500
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Mouse IgG2a heavy chain
Packing Type :
Wet Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.1% (w/v) Sodium Azide
Purity and Specificity :
This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Rabbit Serum.  Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
Stabilizer :
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Storage :
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Synonyms :
Rabbit Anti-Mouse IgG2a (gamma 2a chain) Antibody alkaline phosphatase Conjugated, Rabbit Anti-Mouse IgG2a Antibody alk phos Conjugation
Target Species :
Mouse
Background :
Anti-Mouse IgG2a Alkaline Phosphatase antibody generated in rabbit detects reactivity to Mouse IgG2a (Gamma 2a chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25% of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Low Endotoxin :
No
Sample Size :
No
Secondary Antibody Specificity :
IgG2a
Application Text :
ELISA,Immunohistochemistry,Western Blot,
Category :
Secondary Antibodies
Conjugation Name :
Alkaline Phosphatase (Calf Intestine)
Primary Image Name :
Mouse IgG2a Antibody Alkaline Phosphatase Conjugated - Western Blot
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.