product summary
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company name :
Rockland Immunochemicals
product type :
secondary antibody
product name :
Mouse IgG2b (Gamma 2b chain) Antibody Peroxidase Conjugated
catalog :
610-4342
quantity :
1 mg
more info or order :
Rockland Immunochemicals product webpage
citations: 1
Reference
Krieger J, Stifter K, Riedl P, Schirmbeck R. Cationic domains in particle-forming and assembly-deficient HBV core antigens capture mammalian RNA that stimulates Th1-biased antibody responses by DNA vaccination. Sci Rep. 2018;8:14660 pubmed publisher
product information
Catalog Number :
610-4342
Name :
Anti-MOUSE IgG2b (Gamma 2b chain) (RABBIT) Antibody Peroxidase Conjugated - 610-4342
Display Name :
Mouse IgG2b (Gamma 2b chain) Antibody Peroxidase Conjugated
Application Note :
Anti-Mouse IgG2b peroxidase conjugated antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
1.0 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
HRP
Size :
1 mg
Default Unit :
mg
ELISA Dilution :
1:65,000
Immunohistochemistry Dilution :
1:500 - 1:2,500
Western Blot Dilution :
1:1,000 - 1:5,000
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Anti-Mouse IgG2b was produced by repeated immunization with mouse IgG2b heavy chain in rabbit.
Packing Type :
Ambient
Physical State :
Lyophilized
Preservative :
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Purity and Specificity :
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum.  Specificity was confirmed by ELISA at less than 1% cross-reactivity against other mouse or human heavy or light chain isotypes.
Reconstitution Buffer :
Restore with deionized water (or equivalent)
Reconstitution Volume :
1.0 mL
Species Reactivity :
Mouse
Stabilizer :
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Storage :
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Synonyms :
Rabbit Anti-Mouse IgG2b (gamma 2b chain) Antibody peroxidase Conjugated, Rabbit Anti-Mouse IgG2b Antibody HRP Conjugation
Target Species :
Mouse
Background :
Anti-Mouse IgG2b peroxidase conjugated antibody generated in rabbit detects specifically mouse IgG2b. This secondary peroxidase conjugated antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Immunogen Type :
Native Protein
Low Endotoxin :
No
Sample Size :
No
Secondary Antibody Specificity :
IgG2b
Application Text :
ELISA,Immunohistochemistry,Western Blot,
Other :
User Optimized
Category :
Secondary Antibodies
Conjugation Name :
Peroxidase (Horseradish)
Primary Image Name :
ELISA Results of Rabbit Anti-Mouse IgG2b Antibody Peroxidase Conjugation
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.